An androgen receptor negatively induced long non-coding RNA ARNILA binding to miR-204 promotes the invasion and metastasis of triple-negative breast cancer

Cell Death and Differentiation
Fang YangXiaoxiang Guan

Abstract

Androgen receptor (AR) is emerging as a novel prognostic biomarker in triple-negative breast cancer (TNBC), but the underlying mechanisms remain unknown. As accumulating evidence has shown that long non-coding RNAs (lncRNAs) regulate important cancer hallmarks, we hypothesised that AR-regulated lncRNAs might play roles in TNBC progression. Here, we performed experiments with or without DHT treatment in three TNBC cell lines, and we identified an AR negatively induced lncRNA (ARNILA), which correlated with poor progression-free survival (PFS) in TNBC patients and promoted epithelial-mesenchymal transition (EMT), invasion and metastasis in vitro and in vivo. Subsequently, we demonstrated that ARNILA functioned as a competing endogenous RNA (ceRNA) for miR-204 to facilitate expression of its target gene Sox4, which is known to induce EMT and contribute to breast cancer progression, thereby promoting EMT, invasion and metastasis of TNBC. Our findings not only provide new insights into the mechanisms of lncRNA in regulating AR but also suggest ARNILA as an alternative therapeutic target to suppress metastasis of TNBC patients.

References

Oct 27, 2004·PLoS Biology·Bino JohnDebora S Marks
Sep 17, 2008·Molecular and Cellular Biology·Yan-Nian LiuJi-Hshiung Chen
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Jul 10, 2010·Science·Miao-Chih TsaiHoward Y Chang
Nov 12, 2010·The New England Journal of Medicine·William D FoulkesJorge S Reis-Filho
Sep 20, 2011·Molecular Cell·Kevin C Wang, Howard Y Chang
Sep 25, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Aug 10, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Aye Aye ThikePuay Hoon Tan
Aug 29, 2013·Breast Cancer Research : BCR·Jenny G Parvani, William P Schiemann
Nov 28, 2013·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Dec 25, 2015·Genes & Development·Gayatri ArunDavid L Spector
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
Nov 3, 2016·Breast Cancer Research and Treatment·András LánczkyBalázs Győrffy
Jun 6, 2017·The New England Journal of Medicine·Mark RobsonPierfranco Conte

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Citations

Jan 8, 2019·Clinical Genetics·Carolina MathiasJaqueline C de Oliveira
Mar 1, 2019·Endocrine-related Cancer·Rayzel FernandesLuke A Selth
Apr 16, 2019·Technology in Cancer Research & Treatment·Tianzhu ZhangZhongxin Lu
Mar 2, 2019·Critical Reviews in Clinical Laboratory Sciences·Stella K Vasiliou, Eleftherios P Diamandis
May 9, 2019·Journal of Cellular Physiology·Xiaowei LiYongbin Ding
Sep 5, 2020·Journal of Cellular and Molecular Medicine·Wei XiangGuosong Jiang
Jun 20, 2019·Frontiers in Oncology·Yinan WuSuzhan Zhang
Apr 4, 2019·Frontiers in Genetics·Erika Bandini, Francesca Fanini
Sep 16, 2020·Cell Death & Disease·Lingxia LiuJun Lu
Jan 30, 2019·Journal of Experimental & Clinical Cancer Research : CR·Shuo CaoYixue Xue
Mar 20, 2020·Molecular Cancer·Francesca CrudeleStefano Volinia
Mar 7, 2020·Journal of Cellular and Molecular Medicine·Ziqian SunXiaojun Liu
May 10, 2019·Journal of Mammary Gland Biology and Neoplasia·Gaurav A MehtaMichael L Gatza
Sep 29, 2020·Frontiers in Endocrinology·Mengyao ChenFuming Qiu
Dec 17, 2019·Breast Disease·Nihad Al-OthmanMoath Alqaraleh
Dec 15, 2020·Cell Proliferation·Wenwen ZhangJinhai Tang
Dec 18, 2020·Frontiers in Oncology·Simona Ruxandra VolovatIolanda Augustin
Nov 24, 2020·Journal of Oncology·Mohammad Reza KarimzadehAmirhossein Sahebkar
May 29, 2020·Seminars in Cancer Biology·Kah Keng Wong
Feb 19, 2021·Life Sciences·Jie LiuChen-Yan Zhang
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Yongyong YangQi Cao
Jun 12, 2021·Frontiers in Oncology·Xiaoyong GeXinwei Han
Jun 10, 2021·Journal of Cellular Physiology·Krishan K ThakurAjaikumar B Kunnumakkara
Jun 15, 2021·Frontiers in Oncology·Cecilia ZampedriJorge Melendez-Zajgla
Nov 6, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuyin YiPeter C K Leung

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Methods Mentioned

BETA
ChIP
immunoprecipitation
transfection
RNA-seq
transfections
PCR

Software Mentioned

miRanda
Ensembl
miRanda Plotter
GraphPad Prism
SPSS
ARNILA
Kaplan−Meier Plotter
TargetScan

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