An Anopheles transgenic sexing strain for vector control

Nature Biotechnology
Flaminia CatterucciaAndrea Crisanti

Abstract

Genetic manipulation of mosquito species that serve as vectors for human malaria is a prerequisite to the implementation of gene transfer technologies for the control of vector-borne diseases. Here we report on the development of transgenic sexing lines for the mosquito Anopheles stephensi, the principal vector of human malaria in Asia. Male mosquitoes, expressing enhanced green fluorescent protein (EGFP) under the control of the beta2-tubulin promoter, are identified by their fluorescent gonads in as early as their 3(rd) instar larval stage, and can be efficiently separated from females using both manual methods and automated sorting machines. Importantly, beta2-EGFP males are not impaired in their mating ability and viable fluorescent spermatozoa are also detected in spermathecae of wild-type females mated with transgenic males. The transgenic mosquito lines described here combine most of the features desired and required for a safe application of transgenic methodologies to malaria-control programs.

References

Jan 1, 1992·Medical and Veterinary Entomology·D A LindquistM J Hall
Aug 1, 1985·Journal of Economic Entomology·J D Lines, C F Curtis
Mar 1, 1974·The American Journal of Tropical Medicine and Hygiene·C S LofgrenK F Baldwin
Jan 15, 2000·Insect Molecular Biology·Alfred M Handler, R A Harrell
Jul 6, 2000·Nature·Flaminia CatterucciaAndrea Crisanti
Jan 11, 2001·Development Genes and Evolution·C Horn, Ernst A Wimmer
Feb 15, 2001·Nature Biotechnology·E E FurlongM P Scott
May 30, 2002·Molecular and Biochemical Parasitology·Luke Alphey, Morten Andreasen
Oct 5, 2002·Science·Luke AlpheyFrank H Collins
Aug 7, 2003·Trends in Parasitology·Mark Q Benedict, Alan S Robinson
Oct 9, 1959·Science·E F KNIPLING
Jan 1, 1987·Parasitology Today·E S KrafsurJ E Novy

Citations

Jan 14, 2011·Parasitology Research·Kamaraju RaghavendraAditya P Dash
Jul 20, 2007·Molecular Genetics and Genomics : MGG·Christina ScaliFlaminia Catteruccia
Nov 10, 2007·Parasitology·V H MannPaul J Brindley
Mar 11, 2008·Nature Biotechnology·Elena Angulo, Ben Gilna
Sep 12, 2006·Nature Biotechnology·Bart G J KnolsSamuel K Kemboi
Nov 8, 2005·Nature Biotechnology·P W Atkinson
Apr 5, 2007·Nature Protocols·Neil F LoboFrank H Collins
Oct 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marc F ScheteligErnst A Wimmer
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·J ThailayilFlaminia Catteruccia
Jun 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marc F Schetelig, Alfred M Handler
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anjiang TanYongping Huang
Sep 4, 2009·Vector Borne and Zoonotic Diseases·Luke AlpheyStephen L Dobson
Aug 30, 2007·Nucleic Acids Research·N WindbichlerAndrea Crisanti
May 23, 2012·BMC Evolutionary Biology·Kalle MagnussonN Windbichler
Dec 16, 2009·Malaria Journal·Alan S RobinsonJorge Hendrichs
Dec 16, 2009·Malaria Journal·Philippos Aris PapathanosAndrea Crisanti
Dec 16, 2009·Malaria Journal·Flaminia CatterucciaErnst A Wimmer
Dec 23, 2009·PLoS Biology·Tracey Chapman
Feb 1, 2008·PLoS Neglected Tropical Diseases·Corey L BrelsfoardStephen L Dobson
Aug 1, 2008·Journal of the American Mosquito Control Association·Devi S SumanShri Prakash
Oct 29, 2013·Annual Review of Entomology·Luke Alphey
Jun 20, 2014·Nature Protocols·Emilie PondevilleCatherine Bourgouin
Sep 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tania DottoriniFlaminia Catteruccia
Jun 11, 2014·Nature Communications·Roberto GaliziAndrea Crisanti
Jun 4, 2014·Malaria Journal·Binh V LeRichard H G Baxter
Dec 14, 2011·Annual Review of Entomology·Malcolm J Fraser
Aug 26, 2014·Insect Biochemistry and Molecular Biology·Bo ZhaoAlexander S Raikhel
Oct 29, 2015·Medical and Veterinary Entomology·D AchinkoFlaminia Catteruccia
Jan 29, 2016·Parasites & Vectors·Kim Phuc HoangVinh Sy Le
Nov 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Valentino M GantzAnthony A James
Nov 10, 2012·Insect Biochemistry and Molecular Biology·Christian E OgaugwuErnst A Wimmer
May 4, 2011·Insect Biochemistry and Molecular Biology·Vladimir A Kokoza, Alexander S Raikhel
Nov 25, 2014·Genome Biology·Paolo GabrieliFlaminia Catteruccia
Jun 14, 2008·Insect Biochemistry and Molecular Biology·Zach N AdelmanKevin M Myles
Feb 9, 2008·Trends in Parasitology·D OtrantoRobin B Gasser
Oct 6, 2007·Insect Biochemistry and Molecular Biology·Kirsty C CondonLuke Alphey
Feb 7, 2007·International Journal for Parasitology·Paul J Brindley, Edward J Pearce
Apr 2, 2009·Medical and Veterinary Entomology·Alfred M HandlerS R Skoda
Sep 13, 2008·Medical and Veterinary Entomology·D Otranto, R Wall
Nov 11, 2010·Evolution; International Journal of Organic Evolution·Catherine M WardBruce A Hay
Oct 13, 2009·Parasitology International·Iliano V Coutinho-AbreuMarcelo Ramalho-Ortigao
Aug 15, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jorge MoraesCarlos Logullo
Mar 28, 2006·Trends in Parasitology·Mauro T MarrelliMarcelo Jacobs-Lorena
Jun 15, 2014·Insect Biochemistry and Molecular Biology·Fang LiMaxwell J Scott
Aug 2, 2011·Current Opinion in Microbiology·Eric Marois
Apr 19, 2015·Malaria Journal·James E GentileGregory R Madey
Sep 17, 2013·Insect Biochemistry and Molecular Biology·Sanyuan MaQingyou Xia
Nov 11, 2008·Cell Host & Microbe·Olle TereniusAnthony A James
Aug 4, 2016·Scientific Reports·Roberto GaliziAndrea Crisanti
Apr 25, 2006·BioTechniques·Anthony E Brown, Flaminia Catteruccia
Dec 1, 2018·Biology Open·Alexander NashN Windbichler
Feb 16, 2013·Nature Reviews. Microbiology·Elizabeth A McGraw, Scott L O'Neill
May 23, 2019·G3 : Genes - Genomes - Genetics·Sher Afzal KhanZach N Adelman
Jul 24, 2020·PLoS Genetics·Charlotte Douglas, James M A Turner
Feb 12, 2020·BMC Genomics·Tales Vicari PasciniGustavo Ferreira Martins
Jul 3, 2013·Pathogens and Global Health·Luke AlpheyCamilla Beech
May 2, 2020·Nature Communications·Nikolay P KandulOmar S Akbari
May 19, 2012·Pathogens and Global Health·T A KleinMark Q Benedict
Aug 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zhongjie ZhangYongping Huang
Dec 26, 2018·Parasites & Vectors·Philippos Aris PapathanosAbdoulaye Diabate
Sep 24, 2015·PLoS Biology·Julien Pompon, Elena A Levashina
Dec 26, 2018·Parasites & Vectors·Irina Häcker, Marc F Schetelig

Related Concepts

Enhanced green fluorescent protein
Anopheles Genus
DNA Transposons
Subfertility
Malaria
Immunofluorescence Microscopy
Mosquito Control
Episomes
Sex Determination Analysis
Testis

Related Feeds

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.