An anti-CD44 antibody does not enhance engraftment of DLA-identical marrow after low-dose total body irradiation

Transplant Immunology
Brenda M SandmaierT Graham

Abstract

920 cGy total body irradiation (TBI) is adequate for consistently successful engraftment of marrow from dog leukocyte antigen (DLA)-identical littermates; however, the dose is inadequate to ensure a marrow graft from DLA-nonidentical unrelated donors. Such mismatched grafts are successful only after 1800 cGy, given in three fractions. While anti-T-cell reagents enhance engraftment of DLA-identical littermate marrow after 920 cGy, they fail to be effective in the DLA-nonidentical setting. However, a monoclonal antibody (mAb) to CD44, S5, was found to be very effective in enhancing engraftment of DLA-nonidentical marrow. The current study asked whether mAb S5 was also effective in the setting of DLA-identical littermate transplants. To this purpose, the TBI dose was lowered to 450 cGy, a dose after which 70% of such grafts failed. Four dogs were treated with antibody S5, 0.2 mg/kg on days -7 through -2 (per previously published protocol), given 450 cGy TBI followed by marrow grafts from their DLA-identical littermates. All four dogs rejected their grafts; two of these died from marrow aplasia, and two survived with endogenous marrow recovery. This result was not statistically significantly different from that in 17, historical (n...Continue Reading

References

May 20, 1993·International Journal of Radiation Oncology, Biology, Physics·R StorbM Pepe

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Citations

Nov 19, 2004·Springer Seminars in Immunopathology·Frédéric Baron, Rainer Storb
Jun 23, 1999·Experimental Hematology·S GhaffariC Eaves

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