PMID: 9191081Jun 1, 1997Paper

An anti-chi-1 antibody recognizes a heavily glycosylated protein in rat brain

Brain Research. Molecular Brain Research
A M Ciabarra, K A Sevarino

Abstract

The chi(-1) subunit is a recently identified member of a new class of the ionotropic glutamate receptor family that attenuates NMDA receptor current. We have generated a polyclonal C-terminal antibody to the chi(-1) subunit which recognizes a 135-kDa protein in membranes prepared from chi(-1) transfected HEK-293 cells and in rat brain. In the post-natal day 7 (P7) rat brain, Western blot analysis revealed a 135-kDa band in the thalamus and cortex but not the striatum, cerebellum or peripheral tissues. De-glycosylation of the chi(-1) subunit in both transfected cell lines and in the brain reduced the 135-kDa band to 110 kDa, near the predicted molecular weight of the chi(-1) subunit. These studies demonstrate the chi(-1) subunit is expressed as a glycosylated protein subunit in a distribution that parallels that observed for chi(-1) mRNA by in situ hybridization.

References

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Citations

Sep 5, 2002·The Journal of Comparative Neurology·Hon-Kit WongNikolaus J Sucher
Jul 1, 2004·Journal of Child Neurology·Timothy A SimeoneJong M Rho
Oct 23, 2002·Molecular Pharmacology·Rana A Al-HallaqRobert P Yasuda

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