PMID: 7009750Jan 1, 1980Paper

An anti-human t-lymphocyte antiserum. Further characterization of the specificity for T-cell subpopulations and a comparison of methods for identification of T-lymphocytes in tissue sections

Journal of Immunological Methods
L R Braathen

Abstract

An anti-human T-lymphocyte antiserum has been further studied for specificity for T-cell subpopulations and application in tissue sections. Using a complement-dependent microcytotoxicity assay about 60% of normal peripheral blood T-cells were found to be sensitive to lysis, while 40% were resistant. When the T-cell suspensions were depleted of TG-cells using EA (Ripley) rosette sedimentation, the remaining cells demonstrated an increased percentage of lysis-sensitive cells, while the T-cells enriched in EA-RFC demonstrated a decreased percentage of cells sensitive to lysis, indicating that the antiserum was primarily directed against the non TG-cells, i.e. probably the TM helper cell population. This was further supported by functional studies. In order to quantitate T-cells, cytocentrifuge preparations were made from cell suspensions with known T-cell percentages and the T-cells determined with both the immune adherence technique using AET-treated sheep erythrocytes, as well as the indirect immunofluorescence technique using anti-T antiserum. The results of the two methods correlated well, suggesting that both methods can be used to determine T-cells in situ in infiltrate-like clusters of cells.

References

Jun 1, 1978·The British Journal of Dermatology·J R Bjerke, H K Krogh
May 1, 1979·The British Journal of Dermatology·L R BraathenT Eeg-Larsen
Jun 1, 1979·Clinical and Experimental Dermatology·L R BraathenO J Mellbye
Jun 11, 1968·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E A BoyseL J Old
Jan 1, 1973·Transplantation Reviews·S S Froland, J B Natvig
Jul 1, 1974·Journal of Immunological Methods·M E Kaplan, C Clark

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