PMID: 2118162Aug 28, 1990Paper

An anti-p24 monoclonal antibody shows cross-reactivity with multiple HIV-1 proteins

Journal of Immunological Methods
C M LiangJ S Epstein

Abstract

We produced three murine monoclonal antibodies (mAbs) against the HIV-1 proteins. These three mAbs, namely CA-1, CA-2, CA-4, were IgG1 and all reacted with p24 on the HIV-1 Western blot. One of the mAbs, CA-4, also recognized p13, p21, p28, p29, p32, p39, p47, p55 on the Biotech/Du Pont HIV-1 Western blot strips and p21, p24, p28, p29, p39, p47, p55, p68, p80, p96; p110 on the Bio-Rad strips. CA-4 did not react with H-9 cell lysate nor with other retroviral antigens such as HTLV-1 or HIV-2 proteins. The binding of CA-4 to HIV-1 proteins was not blocked by deglycosylation. All three mAbs reacted with recombinant DNA derived capsid protein (p24) of HIV-1. These results suggest that many proteins in the HIV-1 Western blot contain antigenic epitope(s) similar to that of p24.

References

Jan 24, 1985·Nature·L RatnerK Baumeister
Apr 16, 1985·Biochemical and Biophysical Research Communications·C M LiangT Jost
Jun 13, 1986·Biochemical and Biophysical Research Communications·C M LiangB Allet
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J H Elder, S Alexander

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Citations

Jun 1, 1992·Journal of Medical Virology·L FrankeR von Baehr
May 1, 1992·Clinical and Experimental Immunology·J E Boyd, K James

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