Abstract
Many workers have reported halotolerant bacteria from saline conditions capable of protease production. However, antibiotic resistance and heavy metal tolerance pattern of such organisms is not documented very well. Similarly, only a few researchers have reported the pattern of pH change of fermentation medium during the course of protease production. In this study, we have isolated a halotolerant Bacillus cereus SIU1 strain from a non-saline environment and studied its antibiotic and heavy metal resistance pattern. The isolate produces a thermoalkaline protease and changes the medium pH during the course of fermentation. Thermostability of protease was also studied for 30 min. Seventy bacterial strains isolated from the soils of Eastern Uttar Pradesh, India were screened for protease production. All of them exhibited protease activity. However, 40% bacterial isolates were found good protease producers as observed by caseinolytic zones on milk agar plates. Among them, culture S-4 was adjudged as the best protease producer, and was identified as Bacillus cereus by morphological, biochemical and 16 S rDNA sequence analyses. The isolate was resistant to heavy metals (As2+, Pb2+, Cs1+) and antibiotics (penicillin, lincomycin, cloxa...Continue Reading
References
Jun 1, 1979·Journal of General Microbiology·J ZuccaM T Silva
Feb 1, 1975·Journal of Bacteriology·B N Dancer, J Mandelstam
Jan 1, 1968·Annual Review of Biochemistry·A KornbergM P Deutscher
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·H Neurath
Jun 20, 2002·Applied Microbiology and Biotechnology·R GuptaP Lorenz
Dec 17, 2002·The Journal of General and Applied Microbiology·Keiichi GotoYoshito Sadaie
Jan 1, 1960·Annual Review of Biochemistry·B S HARTLEY
Aug 6, 2004·Microbiological Research·Rathindra Mohan Banik, Monika Prakash
Jul 19, 2005·Applied Microbiology and Biotechnology·Niu QiuhongZhang Keqin
Jan 3, 2006·Journal of Medical Microbiology·Enrico TortoliFrancesco Leoncini
Feb 16, 2006·Letters in Applied Microbiology·T MiyajiN Tomizuka
Apr 10, 2009·Microbial Cell Factories·Randa A AbushamMahiran Basri
Sep 20, 1938·The Journal of General Physiology·M L Anson
Jan 28, 2012·Indian Journal of Microbiology·Manikant TripathiSatyendra K Garg
Citations
Jun 4, 2013·Ecotoxicology and Environmental Safety·Haiou WangGuangrong Zhong
Oct 1, 2013·BMC Microbiology·Xiang ZhouJian-Ping Liang
Jul 14, 2011·BMC Complementary and Alternative Medicine·Vinayak R TripathiSatyendra K Garg
Dec 30, 2011·Microbial Cell Factories·Santosh K SinghSatyendra K Garg
Oct 12, 2013·Applied Biochemistry and Biotechnology·Chittoor Jabeena Thaz, Gurunathan Jayaraman
May 3, 2014·Environmental Science and Pollution Research International·Saurabh GuptaNagaraja Tejo Prakash
Jan 31, 2015·International Journal of Molecular Sciences·Yan ZhouQing-Ping Du
Feb 9, 2016·Environmental Science & Technology·Synthia MaesTom Hennebel
Oct 25, 2013·Preparative Biochemistry & Biotechnology·Muthusamy GovarthananByung-Taek Oh
Apr 14, 2016·Scientific Reports·Han LiuWeimin Wang
Feb 15, 2015·Applied and Environmental Microbiology·Kyle C PeetJanelle R Thompson
Sep 11, 2012·Journal of Basic Microbiology·Deepali BishtNandan Singh Darmwal
Jan 18, 2020·Biotechnology Progress·Amal HammamiMoncef Nasri
Feb 26, 2019·Microbiology Resource Announcements·Olubukola Oluranti BabalolaAyansina Segun Ayangbenro
Sep 23, 2020·Current Microbiology·Derya Efe
Jun 14, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Samaneh AbdollahiFatemeh Shahryari
Jul 1, 2012·Indian Journal of Clinical Biochemistry : IJCB·Mehri Mortazavi, Azim Akbarzadeh
Jul 5, 2017·World Journal of Microbiology & Biotechnology·Viridiana Rodríguez-SánchezLuz Elena Vidales-Rodríguez
Jun 1, 2017·Journal, Genetic Engineering & Biotechnology·Kanupriya Miglani SharmaAshwani Kumar
Jun 7, 2019·Meat Science·Fangda SunQian Liu
Jul 1, 2021·Heliyon·Enoch A AkinpeluJustine O Angadam
Dec 20, 2013·Environmental Science & Technology·Baharak HosseinkhaniNico Boon