An antibody against a conserved C-terminal consensus motif from plant alternative oxidase (AOX) isoforms 1 and 2 label plastids in the explosive dwarf mistletoe (Arceuthobium americanum, Santalaceae) fruit exocarp.

Protoplasma
C M Ross FriedmanG D Martens

Abstract

Dwarf mistletoes, genus Arceuthobium (Santalaceae), are parasitic angiosperms that spread their seeds by an explosive process. As gentle heating triggers discharge in the lab, we wondered if thermogenesis (endogenous heat production) is associated with dispersal. Thermogenesis occurs in many plants and is enabled by mitochondrial alternative oxidase (AOX) activity. The purpose of this study was to probe Arceuthobium americanum fruit (including seed tissues) collected over a 10-week period with an anti-AOX antibody/gold-labeled secondary antibody to determine if AOX could be localized in situ, and if so, quantitatively assess whether label distribution changed during development; immunochemical results were evaluated with Western blotting. No label could be detected in the mitochondria of any fruit or seed tissue, but was observed in fruit exocarp plastids of samples collected in the last 2 weeks of study; plastids collected in week 10 had significantly more label than week 9 (p = 0.002). Western blotting of whole fruit and mitochondrial proteins revealed a signal at 30-36 kD, suggestive of AOX, while blots of whole fruit (but not mitochondrial fraction) proteins showed a second band at 40-45 kD, in agreement with plastid termin...Continue Reading

References

Aug 1, 1995·Journal of Bioenergetics and Biomembranes·D A Day, J T Wiskich
Jul 24, 1998·Archives of Biochemistry and Biophysics·M H Hoefnagel, J T Wiskich
May 4, 1999·FEBS Letters·M E Andersson, P Nordlund
Jan 14, 2001·The Journal of Biological Chemistry·B AntonssonJ C Martinou
Dec 16, 2004·Molecular & Cellular Proteomics : MCP·Karim RezaulDavid Han
Apr 23, 1965·Science·T E Hinds, F G Hawksworth
Sep 18, 2007·Plant Physiology·Maryam ShahbaziMarcel Kuntz
Apr 29, 2008·Biochimica Et Biophysica Acta·Anneke M WagnerAnthony L Moore

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Citations

Feb 11, 2015·Nature Communications·Rolena A J deBruynCynthia Ross Friedman

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