An antibody panel for highly specific detection and differentiation of Zika virus.

Scientific Reports
Md Alamgir KabirWaseem Asghar

Abstract

Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitos. ZIKV can be transmitted from mother to fetus during pregnancy and can cause microcephaly and other birth defects. Effective vaccines for Zika are yet to be approved. Detection of the ZIKV is based on serological testing that often shows cross-reactivity with the Dengue virus (DENV) and other flaviviruses. We aimed to assemble a highly specific anti-Zika antibody panel to be utilized in the development of a highly specific and cost-effective ZIKV rapid quantification assay for viral load monitoring at point-of-care settings. To this end, we tested the affinity and specificity of twenty one commercially available monoclonal and polyclonal antibodies against ZIKV and DENV envelope proteins utilizing nine ZIKV and twelve DENV strains. We finalized and tested a panel of five antibodies for the specific detection and differentiation of ZIKV and DENV infected samples.

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Citations

Apr 20, 2021·Microbes and Infection·Md Alamgir KabirWaseem Asghar
Aug 9, 2021·Talanta·Taís Aparecida Reis CordeiroLucas Franco Ferreira

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Datasets Mentioned

BETA
GTX133314
GTX133325
GTX133326

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
protein assay

Software Mentioned

Clustal Omega

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