An antibody that inhibits fibronectin-independent adhesion of fibroblasts to extracellular matrix material

Journal of Cellular Physiology
P J Kelleher, R L Juliano

Abstract

Chinese hamster ovary (CHO) fibroblasts adhere to the extracellular matrix by both fibronectin-dependent and -independent mechanisms (Harper and Juliano, 1981a,b). Previous studies have suggested that a trypsin-sensitive, 265,000-dalton membrane glycoprotein (gp265) is involved in the fibronectin-independent adhesion process. Using a polyclonal antibody against soluble products obtained from trypsin-treated CHO cells, we have been able to further analyze this involvement. This antibody immunoprecipitates a trypsin-sensitive 265,000-dalton protein from detergent-solubilized cells. Incubation of AdvF11, a variant cell line that does not utilize fibronectin for adhesion, with this antibody blocks their adhesion to extracellular matrix material (ECM). The immunoglobulin fraction will also partially block adhesion of the parental cell line to ECM particularly when the ECM is first treated with an antifibronectin antibody. Taken together these results add support for the involvement of gp265 in fibronectin-independent adhesion and provide a methodology for further characterization.

References

Jul 8, 1977·Biochimica Et Biophysica Acta·V B HatcherF J Yost
Jan 1, 1978·International Review of Cytology·F Grinnell
Sep 21, 1979·Biochimica Et Biophysica Acta·M K Cathcart, L A Culp
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·I Yahara, G M Edelman
Aug 26, 1975·Biochemistry·R L Juliano, M Behar-Bannelier
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G M EdelmanJ L Wang
Feb 1, 1980·The Biochemical Journal·J G Whateley, P Knox
Jun 1, 1984·Experimental Cell Research·M A Schwarz, R L Juliano
Dec 1, 1982·Biochimica Et Biophysica Acta·J D Aplin, R C Hughes
Jan 15, 1981·Developmental Biology·J L MagnaniM S Steinberg
Mar 31, 1980·The Journal of Membrane Biology·G TaroneP M Comoglio
Mar 1, 1981·The Journal of Cell Biology·H K KleinmanG R Martin
Mar 12, 1981·Nature·P A Harper, R L Juliano
Jul 1, 1981·Experimental Cell Research·E Pearlstein, S T Hoffstein

❮ Previous
Next ❯

Citations

Dec 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K KratochwilK Harbers
May 1, 1989·The Journal of Cell Biology·Y N Danilov, R L Juliano
Apr 23, 2009·Biological Reviews of the Cambridge Philosophical Society·P Eckhard Witten, Ann Huysseune
Jul 1, 1985·Journal of Cellular Physiology·M A Schwarz, R L Juliano
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniela GrimmAugusto Cogoli
Aug 1, 1987·Experimental Cell Research·P J Brown, R L Juliano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.