Abstract
A component of adult mammalian central nervous system (CNS) myelin causes collapse of neuronal growth cones and inhibits axonal growth, properties that may be responsible for the lack of regrowth of injured axons in the CNS. The molecules and detailed mechanism through which the inhibitory activity acts are not known. To study the cellular molecules mediating the response to this inhibitor, we have used an in vitro neurite growth inhibition assay to screen a panel of monoclonal antibodies raised against rat neuronal membrane proteins, for clones capable of blocking the response. One monoclonal antibody (10D) neutralized the inhibition of neurite growth seen when primary sympathetic neurons, PC12 cells or NG108-15 cells were grown on inhibitory CNS myelin substrates, but did not promote growth on non-inhibitory substrates. 10D reacted with neuronal cells but not myelin substrate proteins. The antigen recognized by 10D appears to play a role in the interaction between neurons and their growth substrates, and is a novel candidate for a cellular receptor or associated signalling molecule mediating the response to myelin inhibitors.
References
Jan 1, 1979·Methods in Enzymology·E Hawrot, P H Patterson
Nov 16, 1978·Nature·M ShulmanG Köhler
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P NelsonM Nirenberg
Jun 1, 1991·The Journal of Cell Biology·A LochterA Faissner
Jan 1, 1990·Neuron·J A DaviesR J Keynes
Nov 1, 1990·Neuron·B StahlF Bonhoeffer
Jan 18, 1990·Nature·L Schnell, M E Schwab
Oct 1, 1989·The Journal of Cell Biology·P PeshevaM Schachner
Mar 1, 1988·Neuron·P Caroni, M E Schwab
Apr 1, 1988·The Journal of Cell Biology·P Caroni, M E Schwab
Nov 1, 1982·The Journal of Cell Biology·D R ColmanD D Sabatini
Sep 1, 1994·Neuron·G MukhopadhyayM T Filbin
Oct 1, 1994·Neuron·L McKerracherP E Braun
Jan 1, 1993·Annual Review of Neuroscience·M E SchwabC E Bandtlow
Jan 13, 1994·Nature·L SchnellM E Schwab
Oct 22, 1993·Cell·Y LuoJ A Raper
Jan 1, 1993·Science·M IgarashiM C Fishman
Jan 1, 1993·Science·C E BandtlowS B Kater
Citations
Dec 1, 1995·Journal of Neuroscience Methods·A M LozanoA Roach
Oct 4, 2000·Neurobiology of Aging·D B PettigrewK A Crutcher
Feb 13, 1999·The European Journal of Neuroscience·N E SavaskanT Skutella
Oct 14, 2000·Experimental Neurology·P L AndersenD J Schreyer
Dec 11, 2007·Critical Reviews in Biochemistry and Molecular Biology·Twan Lammers, Sara Lavi
May 9, 1998·Neurosurgery·C H Tator
Oct 15, 1998·Molecular and Cellular Neurosciences·M LabesA Roach