An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7

PloS One
Nikhil Baban GhateNripendranath Mandal

Abstract

This report highlights the phytochemical analysis, antioxidant potential and anticancer activity against breast carcinoma of 70% methanolic extract of lichen, Parmotrema reticulatum (PRME). Phytochemical analysis of PRME confirms the presence of various phytoconstituents like alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, tannins, anthraquinones, and ascorbic acid; among which alkaloids, phenols and flavonoids are found in abundant amount. High performance liquid chromatography (HPLC) analysis of PRME revealed the presence of catechin, purpurin, tannic acid and reserpine. Antioxidant activity was evaluated by nine separate methods. PRME showed excellent hydroxyl and hypochlorous radical scavenging as well as moderate DPPH, superoxide, singlet oxygen, nitric oxide and peroxynitrite scavenging activity. Cytotoxicity of PRME was tested against breast carcinoma (MCF-7), lung carcinoma (A549) and normal lung fibroblast (WI-38) using WST-1 method. PRME was found cytotoxic against MCF-7 cells with an IC50 value 130.03 ± 3.11 µg/ml while negligible cytotoxicity was observed on A549 and WI-38 cells. Further flow cytometric study showed that PRME halted the MCF-7 cells in S and G2/M phases and induces apoptosis in d...Continue Reading

References

Apr 1, 1995·Free Radical Research·C A Rice-EvansJ B Pridham
Mar 21, 1998·Toxicologic Pathology·S L Waters, R G Schnellmann
Apr 29, 1998·The Journal of Biological Chemistry·J G PastorinoJ L Farber
Jul 25, 2000·Methods in Enzymology·I E Kochevar, R W Redmond
Mar 10, 2001·BioFactors·M IsemuraM Sazuka
Mar 23, 2001·Journal of Agricultural and Food Chemistry·L S LaiW W Chao
Jun 13, 2001·Biochemical and Biophysical Research Communications·V G ReddyN Singh
Jul 18, 2001·Journal of the American Chemical Society·P K FrederiksenP R Ogilby
Aug 14, 2001·Applied Microbiology and Biotechnology·K Müller
Jan 15, 2002·Cardiology Clinics·X RoucouJ C Martinou
Mar 11, 2003·The Journal of Pharmacy and Pharmacology·Marc KesslerLouis Jung
Jan 25, 1957·Science·R M BURTONJ M VENDITTI
Nov 12, 2003·Archives of Pharmacal Research·Apichart SuksamrarnSomkiat Kongkun
Dec 4, 2003·Current Opinion in Cell Biology·Tomomi Kuwana, Donald D Newmeyer
Jan 28, 2004·Cell·Andrew W Murray
Aug 28, 2004·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M A MosaddikP G Waterman
Feb 1, 2005·Plant Foods for Human Nutrition·L H YaoS S Chen
Sep 1, 2005·Biotechnology Letters·B C BeheraUrmila Makhija
Feb 23, 2008·Advances in Experimental Medicine and Biology·Weimin SunLurong Zhang
Nov 1, 2008·Applied Biochemistry and Biotechnology·Neeraj VermaUrmila Makhija
Dec 11, 2008·BMC Complementary and Alternative Medicine·Bibhabasu HazraNripendranath Mandal
Aug 18, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·N K HondaC Q F Leite
Dec 7, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Weicheng HuMyeong-Hyeon Wang
Mar 31, 2011·Mikrobiologiia·B RankovićD Marić
Sep 15, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Nedeljko T ManojlovicGordana Bogdanovic-Dusanovic
Oct 22, 2011·BMC Complementary and Alternative Medicine·Branislav R RankovićTatjana P Stanojković
Jan 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Muhammad KhanTonghui Ma
Jan 26, 2012·Natural Product Research·Sabira BegumBina Shaheen Siddiqui
Apr 11, 2012·International Journal of Molecular Sciences·Neyres Zinia Taveira de JesusLeonia Maria Batista
May 26, 2012·Phytotherapy Research : PTR·Fabrizia BrisdelliGiuseppe Celenza
Jul 21, 2012·Phytotherapy Research : PTR·Wassim Nasri ShebabyCostantine F Daher
May 21, 2013·Cytotechnology·Nikhil Baban GhateNripendranath Mandal

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Citations

Jul 31, 2016·BMC Complementary and Alternative Medicine·Dipankar ChaudhuriNripendranath Mandal
Jan 1, 2017·Journal of Cancer Prevention & Current Research·Karen L ChenZeynep Madak-Erdogan
Oct 2, 2014·International Journal of Oncology·Xiuyu WangJinhuan Wang
Jan 18, 2020·Biomolecules·Zuzana SolárováPeter Solár
Mar 11, 2020·Expert Opinion on Drug Discovery·Mahshid MohammadiLew Christopher
May 12, 2017·Oxidative Medicine and Cellular Longevity·Vinitha M Thadhani, Veranja Karunaratne
Oct 10, 2019·Anais Da Academia Brasileira De Ciências·Bugrahan EmsenHasan Turkez
Jul 28, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A K ShendgeN Mandal
Oct 27, 2020·Journal of Toxicology and Environmental Health. Part a·Min-Kyung JooYoung-Jin Chun
Feb 13, 2021·Natural Product Research·Thuc-Huy DuongBui-Linh-Chi Huynh
May 31, 2021·Seminars in Cancer Biology·Tanvir Ul Hassan DarShafiul Haque
Jul 5, 2021·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Bugrahan Emsen, Ayse Levent Kolukisa

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Methods Mentioned

BETA
Fluorescence
ELISA
flow cytometry
Assay
xenograft

Software Mentioned

FACSuite
KyPlot
ImageJ

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