An Antisense Circular RNA Regulates Expression of RuBisCO Small Subunit Genes in Arabidopsis.

Frontiers in Plant Science
He ZhangYinglang Wan

Abstract

Circular RNA (circRNA) is a novel class of endogenous long non-coding RNA (lncRNA) and participates in diverse physiological process in plants. From the dataset obtained by high-throughput RNA sequencing, we identified a circRNA encoded by the sense strand of the exon regions spanning two RuBisCO small subunit genes, RBCS2B and RBCS3B, in Arabidopsis thaliana. We further applied the single specific primer-polymerase chain reaction (PCR) and Sanger sequencing techniques to verify this circRNA and named it ag-circRBCS (antisense and across genic-circular RNA RBCS). Using quantitative real-time PCR (qRT-PCR), we found that ag-circRBCS shares a similar rhythmic expression pattern with other RBCS genes. The expression level of ag-circRBCS is 10-40 times lower than the expression levels of RBCS genes in the photosynthetic organs in Arabidopsis, whereas the Arabidopsis root lacked ag-circRBCS expression. Furthermore, we used the delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) to deliver in vitro synthesized ag-circRBCS into Arabidopsis seedlings. Our results indicate that ag-circRBCS could significantly depress the expression of RBCS. Given that ag-circRBCS was expressed at low concentration in vivo, we sugges...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H L SangerA K Kleinschmidt
Mar 1, 1993·Plant Physiology·A DedonderE Krebbers
Dec 6, 2011·Nucleic Acids Research·Miri DananRotem Sorek
Dec 20, 2012·RNA·William R JeckNorman E Sharpless
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Mar 13, 2014·PloS One·Peter L WangJulia Salzman
Sep 23, 2014·RNA·Petar GlažarNikolaus Rajewsky
Dec 3, 2014·RNA·Yang Wang, Zefeng Wang
Jul 25, 2015·The New Phytologist·Chu-Yu YeLongjiang Fan
Jul 28, 2016·Frontiers in Plant Science·Mani K ChoudharyDavid E Somers
Aug 6, 2016·Bioinformatics·Li ChenLongjiang Fan
Aug 12, 2016·Scientific Reports·Tim SchneiderAlbrecht Bindereif
Nov 24, 2016·FEBS Letters·Yudong ShenWeimin Wang
Jan 27, 2017·Annual Review of Plant Biology·Andreas BracherManajit Hayer-Hartl
Mar 21, 2017·Molecular Plant·Qinjie ChuLongjiang Fan
Apr 19, 2017·Trends in Plant Science·Wenlong BaoFrantišek Baluška
Oct 14, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gustavo Caetano-Anollés
Nov 5, 2017·Science China. Life Sciences·Jinping ChengBinglian Zheng
Aug 7, 2018·Plant Science : an International Journal of Experimental Plant Biology·Abozar GhorbaniNeena Mitter
Oct 1, 2018·Journal of Zhejiang University. Science. B·Jian-Zhong XuWei-Guo Zhang
Nov 18, 2018·Briefings in Bioinformatics·Qinjie ChuChu-Yu Ye
May 28, 2019·Plant Signaling & Behavior·Shuai LiuYinglang Wan
Sep 10, 2019·Journal of Experimental Botany·Kana YamadaNicolas Salamin
Nov 5, 2019·Molecular Cell·Sue Mei Tan-WongNick J Proudfoot
Feb 28, 2020·EMBO Reports·František Baluška, Stefano Mancuso
Mar 4, 2020·International Journal of Molecular Sciences·Mao SuganamiAmane Makino
May 31, 2020·Trends in Biochemical Sciences·Manajit Hayer-Hartl, F Ulrich Hartl
Jun 9, 2020·Frontiers in Molecular Biosciences·Peijing ZhangMing Chen
Jul 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Fang HuangLu-Ning Liu
Jul 25, 2020·Journal of Plant Physiology·Susanne von Caemmerer
Aug 5, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yi XuYunfu Cui

❮ Previous
Next ❯

Software Mentioned

SPSS

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.