PMID: 2503790May 22, 1989Paper

An antiserum against amyloid beta-protein precursor detects a unique peptide in Alzheimer brain

Neuroscience Letters
G ColeT Saitoh

Abstract

An antiserum was raised against an amino acid sequence predicted from the DNA sequence of amyloid beta-protein precursor (ABPP), and it was then affinity-purified. This affinity-purified antibody (anti-GID) intensely stained neurons and dystrophic neurites in plaques of Alzheimer's disease (AD) patients, but marginally stained neurons of age-matched normal individuals. Anti-GID antibody detected a series of protein bands with a molecular weight centered at 100,000 and a second band at 55,000 on a blot of the human brain particulate fraction. It also stained a set of bands with a molecular weight around 95,000 and a doublet of Mr 16,000 in the soluble fraction. A band at Mr 35,000 was detected in the soluble fraction prepared from brain tissue of AD patients but not from control brain tissue. A strong immunostaining of AD sections with anti-GID and the presence of a Mr 35,000 band unique to AD might reflect an altered processing of ABPP in AD brains.

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Citations

Jun 15, 1994·Journal of Neuroscience Research·D NagyP D Kushner
Nov 19, 2011·Experimental Brain Research·Christian K E Jung, Jochen Herms
Jul 16, 1990·Biochemical and Biophysical Research Communications·G M ColeT Saitoh
Mar 1, 1991·Neurobiology of Aging·G M ColeT Saitoh
Nov 1, 1995·Neurobiology of Aging·T OkazakiN Mori
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A M Cataldo, R A Nixon
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S A FrautschyG M Cole
Apr 1, 1991·Journal of Neurochemistry·I P ShapiroT Saitoh
Dec 31, 1992·Annals of the New York Academy of Sciences·G M ColeT Saitoh
Jan 22, 1990·Brain Research·D S IimotoT Saitoh
Feb 13, 2001·Neurobiology of Disease·K DitarantoA J Yang
May 7, 1990·Brain Research·F G KlierD Schubert
Jan 31, 1991·Biochemical and Biophysical Research Communications·C R AbrahamP Tempst

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