An apicobasal gradient of Rac activity determines protrusion form and position

Nature Communications
Africa CoutoMarios Georgiou

Abstract

Each cell within a polarized epithelial sheet must align and correctly position a wide range of subcellular structures, including actin-based dynamic protrusions. Using in vivo inducible transgenes that can sense or modify Rac activity, we demonstrate an apicobasal gradient of Rac activity that is required to correctly form and position distinct classes of dynamic protrusion along the apicobasal axis of the cell. We show that we can modify the Rac activity gradient in genetic mutants for specific polarity proteins, with consequent changes in protrusion form and position and additionally show, using photoactivatable Rac transgenes, that it is the level of Rac activity that determines protrusion form. Thus, we demonstrate a mechanism by which polarity proteins can spatially regulate Rac activity and the actin cytoskeleton to ensure correct epithelial cell shape and prevent epithelial-to-mesenchymal transitions.

References

Nov 9, 2001·Annual Review of Genetics·U TepassR Fehon
Dec 5, 2003·Nature·Cyrille De JoussineauDaniel Alexandre
Dec 3, 2004·Molecular Biology of the Cell·Wei YuMirjam M P Zegers
Feb 8, 2005·Current Biology : CB·Joerg BetschingerJuergen A Knoblich
Feb 22, 2005·Nature Cell Biology·Takashi NishimuraKozo Kaibuchi
Feb 22, 2005·Nature Cell Biology·Xinyu Chen, Ian G Macara
May 14, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Teresa Gómez del PulgarJuan Carlos Lacal
Sep 24, 2005·Nature·Frank HsiungThomas B Kornberg
Feb 14, 2006·Nature Cell Biology·Huaye Zhang, Ian G Macara
Aug 23, 2007·Science's STKE : Signal Transduction Knowledge Environment·Stephanie L Gupton, Frank B Gertler
Sep 15, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Fabio Demontis, Christian Dahmann
Nov 17, 2007·Biochimica Et Biophysica Acta·Emeline AssématDominique Massey-Harroche
Dec 10, 2009·Nature Reviews. Molecular Cell Biology·Melissa A ChesaroneBruce L Goode
Dec 17, 2009·Nature Cell Biology·Elena KardashErez Raz
Mar 20, 2010·Nature Reviews. Molecular Cell Biology·Kenneth G Campellone, Matthew D Welch
Jun 10, 2010·Journal of Cell Science·Jason S KingRobert H Insall
Mar 23, 2011·The Journal of Cell Biology·Buzz Baum, Marios Georgiou
Oct 4, 2013·Molecular Biology of the Cell·Hidetoshi GonAkira Kikuchi
Dec 1, 2012·Biomolecular Concepts·Priscilla LoMarios Georgiou
Dec 4, 2014·Small GTPases·Natalie Ann Mack, Marios Georgiou

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Citations

Apr 11, 2018·BMC Biology·Ana-Citlali GradillaSteffen Scholpp
Nov 14, 2019·International Journal of Molecular Sciences·Sergio Casas-Tintó, Marta Portela
Jan 23, 2018·Frontiers in Cellular Neuroscience·Nicolas Tricaud
Feb 21, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J VargasJ Perez-Orive
May 10, 2019·Development·Laura González-MéndezIsabel Guerrero
Jun 21, 2020·Biochimica Et Biophysica Acta. Biomembranes·Covadonga Díaz-DíazFernando Martín-Belmonte
Jul 10, 2021·The Journal of Chemical Physics·Viktoriia SavchenkoVictor Kimberg
Jul 28, 2021·Current Biology : CB·Koki KohashiYasuyuki Fujita
Aug 7, 2021·STAR Protocols·Brenda Canales CoutiñoMarios Georgiou
Oct 15, 2021·The Journal of Cell Biology·Fabian BockRoy Zent

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Methods Mentioned

BETA
GTPases
GTPase
biosensor
FRET

Software Mentioned

Neuralized
Adobe Photoshop
Calculator Plus
Excel
Zen
Gal4
Volocity

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