PMID: 9173882Apr 1, 1997Paper

An apparent association between glycosylphosphatidylinositol-anchored proteins and a sphingolipid in Tetrahymena mimbres

The Biochemical Journal
X Zhang, G A Thompson

Abstract

Sphingolipids are thought to stabilize glycosylphosphatidylinositol (GPI)-anchored protein-rich membrane domains of yeast and polarized higher animal cells during the processing and targeting of these proteins to the plasma membrane. A widely used criterion for identifying the stable sphingolipid- and GPI-anchored protein-enriched membrane domains is the resistance of these lipid-modified proteins to solubilization by the detergent Triton X-100 (TX-100) at low temperature. Surprisingly, there have been no reports of sphingolipid/GPI-anchored protein association in protozoans, despite the fact that these cells contain considerably higher levels of GPI-anchored proteins than does any other organism. We report here the presence in Tetrahymena mimbres of a significant pool of GPI-anchored proteins which resisted extraction by 1% TX-100 at 4 degrees C but not at 37 degrees C. Of the total cellular complement of GPI-anchored proteins, which together accounted for more than 2% of whole-cell protein and were especially enriched in surface membranes, 10% of the major 63kDa component (gpi63) and 23% of a somewhat less abundant component (gpi23) were insoluble in TX-100 at 4 degrees C. A substantial proportion of the cell's only abundant ...Continue Reading

Citations

Apr 10, 2015·European Journal of Protistology·Erhan Aslan, Muhittin Arslanyolu
Aug 23, 2011·Mitochondrion·Yelena Bisharyan, Theodore G Clark
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Mar 30, 2004·Anais Da Academia Brasileira De Ciências·Eliana Barreto-BergterMarcio L Rodrigues
Apr 22, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Masaaki Iwamoto, Richard D Allen
Nov 14, 2003·The Journal of Biological Chemistry·Agnieszka E TopolskaRoss L Coppel
Oct 3, 2001·Physiological Reviews·J C HolthuisG Van Meer
Mar 4, 2010·Journal of Cell Science·Ping YiEric Chevet

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