An application in cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad-spectrum bacteriocin, lacticin 3147.

Applied and Environmental Microbiology
M P RyanR P Ross

Abstract

Lactococcus lactis DPC3147, a strain isolated from an Irish kefir grain, produces a bacteriocin with a broad spectrum of inhibition. The bacteriocin produced is heat stable, particularly at a low pH, and inhibits nisin-producing (Nip+) lactococci. On the basis of the observation that the nisin structural gene (nisA) does not hybridize to DPC3147 genomic DNA, the bacteriocin produced was considered novel and designated lacticin 3147. The genetic determinants which encode lacticin 3147 are contained on a 63-kb plasmid, which was conjugally mobilized to a commercial cheese starter, L. lactis subsp. cremoris DPC4268. The resultant transconjugant, DPC4275, both produces and is immune to lacticin 3147. The ability of lacticin 3147-producing lactococci to perform as cheddar cheese starters was subsequently investigated in cheesemaking trials. Bacteriocin-producing starters (which included the transconjugant strain DPC4275) produced acid at rates similar to those of commercial strains. The level of lacticin 3147 produced in cheese remained constant over 6 months of ripening and correlated with a significant reduction in the levels of nonstarter lactic acid bacteria. Such results suggest that these starters provide a means of controllin...Continue Reading

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Citations

Mar 15, 2000·Journal of Food Protection·A G ScannellE K Arendt
Jan 7, 2003·Applied and Environmental Microbiology·Paul D CotterR Paul Ross
Jun 6, 2003·Applied and Environmental Microbiology·Lisa O'SullivanColin Hill
Sep 8, 2006·Applied and Environmental Microbiology·Y KourkoutasA A Koutinas
Jun 24, 2008·Critical Reviews in Biotechnology·Antonio GalvezNabil Ben Omar
Sep 16, 2008·Applied and Environmental Microbiology·Anneke KuipersGert N Moll
May 19, 2009·Applied Biochemistry and Biotechnology·Athanasios A KoutinasMagdalini Soupioni
Dec 1, 2009·Nature Reviews. Microbiology·Michael E HibbingS Brook Peterson
Nov 26, 2009·Applied and Environmental Microbiology·Dagim Jirata BirriIngolf F Nes
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mary C ReaR Paul Ross
Nov 1, 2012·Food & Function·Christopher Beermann, Julia Hartung
Nov 28, 2012·Biochemical Society Transactions·Mohammad R IslamKenji Sonomoto
Mar 29, 2014·Probiotics and Antimicrobial Proteins·Andrea LaukováRudolf Zitňan
Apr 5, 2015·Applied and Environmental Microbiology·Paula M O'ConnorColin Hill
Apr 11, 2015·BioMed Research International·Juan L ArquésMargarita Medina
May 21, 2016·Frontiers in Microbiology·Benjamin C T BourriePaul D Cotter
Sep 24, 2016·Journal of Clinical Medicine·Eamonn P Culligan, Roy D Sleator
Sep 6, 2000·Journal of Applied Microbiology·M C Martínez-CuestaT Requena
Jun 17, 2008·Applied and Environmental Microbiology·Leah A Martin-VisscherJohn C Vederas
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Jun 30, 2009·Applied and Environmental Microbiology·Máire BegleyR Paul Ross
Aug 11, 2010·FEMS Microbiology Reviews·Hikmate AbriouelAntonio Gálvez
Oct 23, 2010·Journal of Basic Microbiology·Han-Sheng GongHui Wang
Oct 29, 2009·Applied Microbiology and Biotechnology·Shashi Bala PrasadK B Ramachandran

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