An approach to assay calcineurin activity and the inhibitory effect of zinc ion

Analytical Biochemistry
Junyi HuangGenxi Li

Abstract

In this study, an electrochemical method to assay calcineurin activity is proposed. Although the enzyme could not exhibit electron transfer reactivity and the catalytic reaction of the substrate could not give any electrochemical wave, p-nitrophenol as the catalytic reaction production could be oxidized at the calcineurin/Triton X-100 film modified electrode to exhibit useful wave that might be employed to assay the enzyme activity. The effect of Ni(2+) and Zn(2+) on calcineurin was also investigated. Whereas Ni(2+) was confirmed to be able to enhance the enzymatic activity, Zn(2+) was found to be an inhibitor to calcineurin.

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Citations

Jun 16, 2012·Nature Reviews. Immunology·Stefan FeskeMurali Prakriya
Apr 30, 2009·Annual Review of Nutrition·Hajo Haase, Lothar Rink
Apr 30, 2009·Journal of Leukocyte Biology·Tolunay B AydemirRobert J Cousins
Mar 25, 2015·Molecular Immunology·Tsukasa UgajinToshio Hirano
Jan 5, 2016·Metallomics : Integrated Biometal Science·K A Taylor, N Pugh
Oct 25, 2017·International Journal of Molecular Sciences·Martina MaywaldLothar Rink
Dec 1, 2017·Nutrients·Inga WesselsLothar Rink
Nov 23, 2016·Journal of Immunology Research·Shintaro Hojyo, Toshiyuki Fukada
Jan 1, 2019·Journal of Immunology Research·Keigo Nishida, Ryota Uchida

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