PMID: 2484379Jan 1, 1989Paper

An approach to assessing trace element bioavailability from milk in vitro. Extrinsic labeling and proteolytic degradation

Biological Trace Element Research
B Lönnerdal, C Glazier

Abstract

Both in vitro and in vivo, the use of a radioisotope can significantly enhance the sensitivity of methods for trace element studies. An essential prerequisite for this approach, however, is that the added (extrinsic) radiolabel equilibrates with the native (cold) element within all compartments of the diet. By using ultracentrifugation, ultrafiltration, and gel filtration chromatography, we have shown that the method is valid for zinc, copper, and manganese when using milks and formulas. For iron, however, extrinsic labeling does not necessarily yield results similar to the native distribution. We have used extrinsic labeling to follow the distribution of Zn, Cu, and Mn between high molecular weight compounds (proteins) and low molecular weight complexes in human and bovine milk after in vitro proteolysis. Peptic digestion at various pHs and pancreatic digestion for varying times were used to mimic digestion in the infant. After limited proteolysis, a large proportion of trace minerals in human milk was found in the low molecular weight fraction, whereas in cow's milk a large proportion was bound to incompletely digested casein. These findings may, at least in part, explain the higher bioavailability of trace elements from huma...Continue Reading

References

Oct 1, 1987·Proceedings of the Society for Experimental Biology and Medicine·R E SerfassR S Houk
Sep 1, 1987·Acta paediatrica Scandinavica·L A Davidson, B Lönnerdal
Feb 1, 1986·Journal of Nutritional Science and Vitaminology·R SatoH Naito
Mar 1, 1985·The American Journal of Clinical Nutrition·B LönnerdalL S Hurley
Nov 1, 1985·The American Journal of Clinical Nutrition·B LönnerdalC L Keen
Jan 1, 1972·Biology of the Neonate·E M WiddowsonR D Milner
Jan 1, 1983·The Journal of Pediatrics·E LebenthalL A Heitlinger
Nov 1, 1984·The American Journal of Clinical Nutrition·B LönnerdalB Sandström
Sep 1, 1983·The American Journal of Clinical Nutrition·B SandströmB Lönnerdal
Feb 1, 1984·The American Journal of Clinical Nutrition·G B Fransson, B Lönnerdal
Jan 1, 1981·Annual Review of Nutrition·B LönnerdalL S Hurley
Dec 1, 1982·The American Journal of Clinical Nutrition·B LönnerdalL S Hurley
Jan 1, 1980·Journal of Inorganic Biochemistry·B LönnerdalL S Hurley
Mar 1, 1980·The Journal of Pediatrics·G B Fransson, B Lönnerdal
Nov 1, 1980·The Journal of Nutrition·H M Mykkänen, R H Wasserman

❮ Previous
Next ❯

Citations

Apr 3, 2014·Nutrition Reviews·Michael R La FranoBetty J Burri
Nov 26, 2010·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Caroline J ChantryBarbara Abrams
Jul 22, 2004·Journal of Agricultural and Food Chemistry·E PeñaP Bermejo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved