An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase

The Plant Cell
T UedaA Nakańo

Abstract

The Arabidopsis Ara proteins belong to the Rab/Ypt family of small GTPases, which are implicated in intracellular vesicular traffic. To understand their specific roles in the cell, it is imperative to identify molecules that regulate the GTPase cycle. Such molecules have been found and characterized in animals and yeasts but not in plants. Using a yeast system, we developed a novel method of functional screening to detect interactions between foreign genes and identified this Rab regulator in plants. We found that the expression of the ARA4 gene in yeast ypt mutants causes exaggeration of the mutant phenotype. By introducing an Arabidopsis cDNA library into the ypt1 mutant, we isolated a clone whose coexpression overcame the deleterious effect of ARA4. This gene encodes an Arabidopsis homolog of the Rab GDP dissociation inhibitor (GDI) and was named AtGDI1. The expression of AtGDI1 complemented the yeast sec19-1 (gdi1) mutation. AtGDI1 is expressed almost ubiquitously in Arabidopsis tissues. The method described here indicates the physiological interaction of two plant molecules, Ara4 and GDI, in yeast and should be applicable to other foreign genes.

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Citations

Apr 28, 2005·Plant Physiology and Biochemistry : PPB·Ivanka Asparuhova Yaneva, Karsten Niehaus
Oct 13, 2000·The Plant Journal : for Cell and Molecular Biology·M YamaguchiH Uchimiya
Jan 24, 2004·Journal of Experimental Botany·Isabelle Chérel
Mar 20, 2003·Plant Physiology·Vanessa VernoudErik Nielsen
Aug 6, 2008·Plant Physiology·Erik NielsenTakashi Ueda
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·M UmedaC Koncz
Oct 25, 2011·Biochemical and Biophysical Research Communications·Jae Bok HeoJeong Dong Bahk
Apr 8, 2009·International Review of Cell and Molecular Biology·Chieko Saito, Takashi Ueda
Oct 29, 2004·Plant & Cell Physiology·Gil-Je LeeInhwan Hwang
Oct 29, 2003·Plant & Cell Physiology·Nobutaka MitsudaMasa H Sato
Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Yuji IwataNozomu Koizumi

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