PMID: 9807817Nov 10, 1998Paper

An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506

The Plant Journal : for Cell and Molecular Biology
J D FaureS H Howell

Abstract

AtFKBP12 is an Arabidopsis cDNA that encodes a protein similar to the mammalian immunophilin, FKBP12. AtFKBP12 was used as 'bait' in a yeast 2-hybrid system to screen for cDNAs in Arabidopsis encoding proteins that bind to FKBP12. Two partial cDNAs were recovered encoding the C-terminus of a protein we have called Arabidopsis thaliana FKBP12 interacting protein 37 (AtFIP37). AtFIP37 is similar to a mammalian protein, FAP48, that also binds to FKBP12. The interaction between AtFKBP12 and AtFIP37 in the 2-hybrid system, as assessed by histidine auxotrophy and beta-galactosidase activity, was disrupted by FK506, but not by cyclosporin A, a drug that binds to cyclophilin A. AtFIP37 was also shown to bind in vitro to AtFKBP12 in GST-fusion protein binding assays. The binding was abolished by prior incubation of AtFKBP12 with FK506. These findings indicate that an Arabidopsis FKBP12 ortholog encodes a protein that binds FK506 and that the interaction between AtFKBP12 and AtFIP37 may involve the FK506 binding site of AtFKBP12. The interaction provides interesting new opportunities for controlling protein:protein interactions in vivo in plants.

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Citations

Sep 22, 2009·Plant Molecular Biology·Peter J Gollan, Mrinal Bhave
Oct 31, 2009·Plant Molecular Biology·David MeiriAdina Breiman
Sep 7, 2001·Trends in Plant Science·Y HarrarJ D Faure
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Thilo KamphausenJens-U Rahfeld
Mar 31, 2004·Plant Physiology·Patrick G N RomanoJulie E Gray
Apr 16, 2004·Plant Physiology·Patrick RomanoSheng Luan
Nov 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rajeev GuptaSheng Luan
Mar 5, 2016·Plant Science : an International Journal of Experimental Plant Biology·Markus GeislerMaria Ivanchenko
Sep 11, 2007·Trends in Plant Science·Markus Geisler, Aurélien Bailly
May 5, 2005·The New Phytologist·Patrick RomanoSheng Luan
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·Jeffrey J EschM David Marks
Oct 21, 2015·Frontiers in Plant Science·Sang S LeeHye S Cho
May 14, 2016·Journal of Integrative Plant Biology·Alice BurgessIain Robert Searle
Apr 27, 2005·Journal of Experimental Botany·Bob B Buchanan, Sheng Luan
Oct 30, 2019·The Plant Journal : for Cell and Molecular Biology·Gloria Serrano-BuenoFederico Valverde
Nov 26, 2020·International Journal of Molecular Sciences·Ming-Yan CheungHon-Ming Lam

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