An ARGS-aggrecan assay for analysis in blood and synovial fluid

Osteoarthritis and Cartilage
S LarssonAndré Struglics

Abstract

To validate a modified ligand-binding assay for the detection of aggrecanase generated aggrecan fragments with the ARGS neoepitope in synovial fluid (SF) and blood, and to verify the identity of aggrecan fragments found in blood. An enzyme-linked immunosorbent assay (ELISA) on the Meso Scale Discovery (MSD) platform for detection of ARGS-aggrecan was validated, using a standard made from recombinant human aggrecan. Matched samples of SF, serum, plasma, and urine were obtained from 36 subjects at different time points after knee injury, and analysed for ARGS-aggrecan content. Aggrecan was purified from serum and plasma pools and analysed by Western blot. The limits of quantification for the ARGS-aggrecan assay was between 0.2 and 0.025 pmol ARGS/ml, and the sensitivity of the assay was improved two-fold compared to when using a standard purified from human donors. The ARGS concentrations were highest in SF (mean, range; 3.02, 0.36-30.22 pmol/ml), 20 times lower in the blood samples (0.14, 0.055-0.28 pmol/ml serum and 0.13, 0.053-0.28 pmol/ml plasma), and 80 times lower in urine (0.036, below detection - 0.087 pmol/ml). Serum-ARGS and plasma-ARGS concentrations were similar, and correlated (r(S) = 0.773, P < 0.001). SF concentrat...Continue Reading

References

Nov 1, 1989·Arthritis and Rheumatism·L S LohmanderD Heinegård
Jan 1, 1984·Clinical and Experimental Pharmacology & Physiology·J R FraserU G Laurent
Sep 1, 1981·The Biochemical Journal·Z Gunja-Smith, R J Boucek
Mar 24, 1999·Arthritis and Rheumatism·L S LohmanderA R Poole
May 30, 2008·BMC Musculoskeletal Disorders·Jean C RousseauMorten A Karsdal
Sep 12, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·André Struglics, Staffan Larsson

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Citations

Dec 29, 2015·Osteoarthritis and Cartilage·A C Bay-JensenC S Thudium
May 25, 2015·Osteoarthritis and Cartilage·S RobertsJ H Kuiper
Dec 19, 2019·Expert Review of Molecular Diagnostics·Anne Christine Bay-JensenMorten Asser Karsdal
Nov 9, 2018·International Journal of Molecular Sciences·Yunyun LuoAnne-Christine Bay-Jensen
Aug 23, 2020·International Journal of Molecular Sciences·Anne Sofie SiebuhrSven Lindemann
Dec 16, 2017·Biotribology·Robert L TrevinoMarkus A Wimmer

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