An artificial 'haemophilic' plasma for one-stage factor-VIII assay

British Journal of Haematology
V ChantarangkulS C Darby


An 'artificial' plasma for one-stage factor-VIII assays is made by incubating human plasma with EDTA, to destroy factor VIII, and afterwards removing the anticoagulant by dialysis. Bovine factor V is then added to a given level. In the assay, contact activation is controlled by adding contact product. It was confirmed that factor-VIII activity was destroyed and that the EDTA was freely dialysable. The fibrinogen in the treated plasma clotted normally with thrombin. Likely variation in the factor-V activity was found not to be critical. The concentration of fibrinogen and other factors was adequate. Variation between batches was small. The artificial plasma yielded assay results closely comparable to haemophilic plasma in samples with factor-VIII activities in the range 0.01--20.0 iu/ml; the mean results in the artificial system were estimated to be 0.997 x those in haemophilic plasma, with a 95% confidence interval of 0.901--1.103. Biological variability in individual assays was smaller in the artificial system than when haemophilic plasma was used. Instability at the bench was more often detected in the artificial system than in haemophilic plasma assays, but the effect was eliminated from the results by obtaining duplicated r...Continue Reading


Jul 1, 1975·Journal of Clinical Pathology·J L Pérez-Requejo, G I Ingram
Sep 1, 1975·British Journal of Haematology·K N WilliamsG I Ingram
Oct 1, 1971·British Journal of Haematology·P F Bruning, E A Loeliger
Jul 1, 1967·Journal of Clinical Pathology·E M Essien, G I Ingram
Aug 1, 1952·The Biochemical Journal·I C INGRAM
Jun 1, 1957·Acta Haematologica·Y BOUNAMEAUX
Oct 1, 1964·British Journal of Haematology·J Margolis, S BRUCE
May 1, 1946·Proceedings of the Society for Experimental Biology and Medicine·G GOMORI


Jun 1, 1992·British Journal of Haematology·C F HirstL Poller
Jul 1, 1996·Haemophilia : the Official Journal of the World Federation of Hemophilia·A FarrugiaM Poulis
Sep 1, 1988·Journal of Clinical Pathology·A G BennyE W Berry
Feb 1, 1981·British Journal of Haematology·D S McLellanA Aronstam
May 1, 1980·British Journal of Haematology·L HollandD G Chalmers
Jan 1, 1987·Pathology·P A OckelfordD G Woodfield
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·J Over
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·J Over
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·S Rosén

Related Concepts

Blood Coagulation Tests
Physical Dialysis
Dose-Response Relationship, Drug
Edetic Acid, Calcium, Sodium Salt
Factor V
Factor VIII Procoagulant Activity
Freeze Drying
Factor Viii Deficiency
Study, Methodological

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