An artificial 'haemophilic' plasma for one-stage factor-VIII assay

British Journal of Haematology
V ChantarangkulS C Darby

Abstract

An 'artificial' plasma for one-stage factor-VIII assays is made by incubating human plasma with EDTA, to destroy factor VIII, and afterwards removing the anticoagulant by dialysis. Bovine factor V is then added to a given level. In the assay, contact activation is controlled by adding contact product. It was confirmed that factor-VIII activity was destroyed and that the EDTA was freely dialysable. The fibrinogen in the treated plasma clotted normally with thrombin. Likely variation in the factor-V activity was found not to be critical. The concentration of fibrinogen and other factors was adequate. Variation between batches was small. The artificial plasma yielded assay results closely comparable to haemophilic plasma in samples with factor-VIII activities in the range 0.01--20.0 iu/ml; the mean results in the artificial system were estimated to be 0.997 x those in haemophilic plasma, with a 95% confidence interval of 0.901--1.103. Biological variability in individual assays was smaller in the artificial system than when haemophilic plasma was used. Instability at the bench was more often detected in the artificial system than in haemophilic plasma assays, but the effect was eliminated from the results by obtaining duplicated r...Continue Reading

References

Jul 1, 1975·Journal of Clinical Pathology·J L Pérez-Requejo, G I Ingram
Sep 1, 1975·British Journal of Haematology·K N WilliamsG I Ingram
Oct 1, 1971·British Journal of Haematology·P F Bruning, E A Loeliger
Jul 1, 1967·Journal of Clinical Pathology·E M Essien, G I Ingram
Aug 1, 1952·The Biochemical Journal·I C INGRAM
Jun 1, 1957·Acta Haematologica·Y BOUNAMEAUX
Oct 1, 1964·British Journal of Haematology·J Margolis, S BRUCE
May 1, 1946·Proceedings of the Society for Experimental Biology and Medicine·G GOMORI

Citations

Jun 1, 1992·British Journal of Haematology·C F HirstL Poller
Jul 1, 1996·Haemophilia : the Official Journal of the World Federation of Hemophilia·A FarrugiaM Poulis
Sep 1, 1988·Journal of Clinical Pathology·A G BennyE W Berry
Feb 1, 1981·British Journal of Haematology·D S McLellanA Aronstam
May 1, 1980·British Journal of Haematology·L HollandD G Chalmers
Jan 1, 1987·Pathology·P A OckelfordD G Woodfield
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·J Over
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·J Over
Jan 1, 1984·Scandinavian Journal of Haematology. Supplementum·S Rosén

Related Concepts

Blood Coagulation Tests
Physical Dialysis
Dose-Response Relationship, Drug
Edetic Acid, Calcium, Sodium Salt
Factor V
Factor VIII Procoagulant Activity
Gamma-Fibrinogen
Freeze Drying
Factor Viii Deficiency
Study, Methodological

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.