An assay to evaluate the long-term effects of inflammatory mediators on murine airway smooth muscle: evidence that TNFalpha up-regulates 5-HT(2A)-mediated contraction

British Journal of Pharmacology
M AdnerLars-Olaf Cardell

Abstract

1. Asthma research is arguably limited by an absence of appropriate animal models to study the pharmacology of inflammatory mediators that affect airway hyperresponsiveness and remodelling. Here we assessed an assay based on mouse tracheal segments cultured for 1-32 days, and investigated contractile responses mediated by muscarinic and 5-hydroxytryptamine (5-HT) receptors following long-term exposure to tumour necrosis factor-alpha (TNFalpha). 2. Following culture, in the absence of TNFalpha, maximum contractile responses to KCl and carbachol were similar, with an increase in response up to day two and a decrease to a stable level after 8 days. Maximal relaxations to isoprenaline were not affected by the culture procedure. The potency of KCl and isoprenaline increased throughout the study. DNA microarray data revealed that global gene expression changes were greater when tissues were introduced to culture than when they were maintained in culture. The morphology of smooth muscle cells was maintained throughout the culture period. 3. 5-HT induced a weak contraction in both fresh and cultured (up to 8 days) segments. Culture with TNFalpha produced a time- and concentration-dependent increase in the maximal contraction to 5-HT, e...Continue Reading

References

Apr 26, 2005·Respiratory Physiology & Neurobiology·O BacharLars-Olaf Cardell
Jun 17, 2011·International Immunology·Yan CaiLars Olaf Cardell
Apr 11, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shannon R MurphyLaura S Van Winkle
Jan 27, 2007·American Journal of Respiratory Cell and Molecular Biology·Yaping ZhangLars-Olaf Cardell
Sep 18, 2007·American Journal of Respiratory Cell and Molecular Biology·Caroline MorinEric Rousseau
Jan 2, 2014·Toxicology and Applied Pharmacology·Yuan Xu, Lars-Olaf Cardell
Mar 29, 2014·American Journal of Respiratory Cell and Molecular Biology·Yuan XuLars-Olaf Cardell
Oct 6, 2005·Experimental Lung Research·Caroline MorinJoseph Brayden
Jul 14, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·O BacharL-O Cardell
Aug 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M BrybornL-O Cardell
Sep 7, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y ZhangL-O Cardell
May 4, 2013·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Elena Alexeevna GolikovaSergey Vitalievich Sennikov
May 4, 2007·Current Opinion in Pharmacology·Mike BerryAj Wardlaw
Jul 9, 2011·Memórias do Instituto Oswaldo Cruz·Suellen D'Arc dos Santos Oliveira, Claudia Lucia Martins Silva
Oct 13, 2004·Pulmonary Pharmacology & Therapeutics·Tanna J FerraraAbdellaziz Ben-Jebria
Nov 1, 2008·Expert Review of Clinical Immunology·Brandie L Walker, Richard Leigh
Jan 12, 2010·The Journal of Pharmacology and Experimental Therapeutics·M AdnerAnna Miller-Larsson
May 9, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ofir BacharLars-Olaf Cardell
Sep 29, 2011·British Journal of Pharmacology·R MakwanaC Page
Oct 9, 2019·Journal of Clinical Medicine·Efthymia TheofaniGeorgina Xanthou
May 21, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Thomas K AnsellPeter B Noble

Citations

May 17, 1976·Biochemical and Biophysical Research Communications·J D FunkhouserR D Peterson
Feb 1, 1992·The American Review of Respiratory Disease·J C KipsR A Pauwels
Nov 1, 1990·Journal of Pharmacological Methods·J GarssenF P Nijkamp
Jan 1, 1990·British Journal of Pharmacology·P J Henry, R G Goldie
Jul 1, 1995·American Journal of Respiratory and Critical Care Medicine·P S ThomasP J Barnes
Nov 1, 1993·The American Journal of Physiology·M J RogersB Horowitz
May 1, 1993·British Journal of Pharmacology·J GarssenF P Nijkamp
Jun 1, 1996·The Journal of Clinical Investigation·H HakonarsonM M Grunstein
Jan 1, 1997·Life Sciences·J ZaagsmaH Meurs
Jul 1, 1997·Canadian Journal of Physiology and Pharmacology·X MaN L Stephens
Dec 2, 1998·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·A HessO Michel
Jul 17, 1999·The American Journal of Physiology·A LindqvistP Hellstrand
Apr 11, 2000·The Journal of Allergy and Clinical Immunology·D Schmidt, K F Rabe
May 12, 2000·American Journal of Respiratory and Critical Care Medicine·J BousquetA M Vignola
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·S J HirstT H Lee
Jan 12, 2001·The European Respiratory Journal·P RytiläT Haahtela
Feb 24, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·I Szelenyi
Apr 18, 2001·International Archives of Allergy and Immunology·A B TonnelI Tillie-Leblond
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·K A SimmenK Früh
Feb 20, 2002·JAMA : the Journal of the American Medical Association·David B Jacoby

Related Concepts

Airway Remodeling
Receptor, Serotonin,5-HT2A
Serotonin Effect
DNA Microarray Format
Tumor Necrosis Factor-alpha
Carboptic
Mice, Inbred BALB C
Organ Culture Techniques
Inflammation Mediators
Murine

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.