An association of 27- and 40-kDa molecules with glycolipids that bind A-B bacterial enterotoxins to cultured cells.

Biochimica Et Biophysica Acta
Takeshi ShimizuHideo Hayashi

Abstract

It is well recognized that the Shiga-like toxins (Stxs) preferentially bind to Gb3 glycolipids and the cholera toxin (CT) and heat-labile enterotoxin (LTp) bind to GM1 gangliosides. After binding to the cell surface, A-B bacterial enterotoxins have to be internalized by endocytosis. The transport of the toxin-glycolipid complex has been documented in several manners but the actual mechanisms are yet to be clarified. We applied a heterobifunctional cross-linker, sulfosuccinimidyl-2-(p-azidosalicylamido)-1,3'-dithiopropionate (SASD), to detect the membrane proteins involved in the binding and the transport of A-B bacterial enterotoxins in cultured cells. Both Stx1 and Stx2 bound to the detergent-insoluble microdomain (DIM) of Vero cells and Caco-2 cells, which were susceptible to the toxin, but neither was bound to insusceptible CHO-K1 cells. Both CT and LTp bound to the DIM of Vero cells, Caco-2 cells, and CHO-K1 cells. In a cross-linking experiment, Stx1 cross-linked only with a 27-kDa molecule, while Stx2, which was more potently toxic than Stx1, cross-linked with 27- and 40-kDa molecules of Vero cells as well as of Caco-2 cells; moreover, no molecules were cross-linked with the insusceptible CHO-K1 cells. LTp was cross-linked...Continue Reading

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Citations

Dec 24, 2005·Molecular Biology of the Cell·Silje Ugland LauvrakKirsten Sandvig
Apr 22, 2009·Infection and Immunity·Takeshi ShimizuMasatoshi Noda
Sep 27, 2006·Infection and Immunity·Luz P Blanco, Victor J Dirita
Jul 30, 2010·PloS One·Audrun UtskarpenKirsten Sandvig
Mar 13, 2012·Microbial Pathogenesis·Kaisar A TalukderAlam Nur-E-Kamal
Aug 16, 2005·The FEBS Journal·Maria L TorgersenKirsten Sandvig
Oct 18, 2006·Biology of the Cell·Blandine Geny, Michel R Popoff
Nov 9, 2010·FEMS Immunology and Medical Microbiology·Maria L TorgersenKirsten Sandvig
Dec 22, 2009·Nature Reviews. Microbiology·Ludger Johannes, Winfried Römer
Nov 19, 2004·The EMBO Journal·Nicole C KestyMeta J Kuehn
May 29, 2007·Microbial Pathogenesis·Takeshi ShimizuTakashi Hamabata

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