An asymmetric sheath controls flagellar supercoiling and motility in the leptospira spirochete.

ELife
Kimberley H GibsonCharles V Sindelar

Abstract

Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations of the entire cell. Motility is powered by the rotation of supercoiled 'endoflagella' that wrap around the cell body, confined within the periplasmic space. To investigate the structural basis of flagellar supercoiling, which is critical for motility, we determined the structure of native flagellar filaments from the spirochete Leptospira by integrating high-resolution cryo-electron tomography and X-ray crystallography. We show that these filaments are coated by a highly asymmetric, multi-component sheath layer, contrasting with flagellin-only homopolymers previously observed in exoflagellated bacteria. Distinct sheath proteins localize to the filament inner and outer curvatures to define the supercoiling geometry, explaining a key functional attribute of this spirochete flagellum.

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Citations

Apr 9, 2020·Biomolecules·Shuichi Nakamura
Aug 28, 2020·Frontiers in Immunology·Marion HolzapfelCatherine Werts
Nov 4, 2020·Biomolecules·Brittany L Carroll, Jun Liu
Jan 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Weili ZhengTiago R D Costa
Oct 22, 2021·PLoS Pathogens·Jenifer CoburnDavid A Haake

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BETA
LIC11890

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BETA
X-ray
pull-down

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SITUS map2map
Rosetta
fscGoldSplitOnTomos
3dautorefine
CTFFind4
addModPts
Relion
MolProbity
Tomo3D
XDS

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