PMID: 9555899Apr 29, 1998Paper

An AT-rich tract containing an integration host factor-binding domain and two UP-like elements enhances transcription from the pilEp1 promoter of Neisseria gonorrhoeae

Journal of Bacteriology
J A Fyfe, J K Davies

Abstract

The pilE gene of Neisseria gonorrhoeae is transcribed from a sigma70 promoter (pilEp1) with an AT-rich tract extending 65 nucleotides upstream of the -35 box. Within this region is an integration host factor (IHF)-binding core consensus sequence. We have performed a detailed analysis to determine which upstream sequences are required for efficient transcription from pilEp1 in N. gonorrhoeae. Deletion of sequences upstream of the AT-rich tract had no effect on the level of transcription. However, the IHF-binding core consensus sequence and the AT-rich sequence further upstream were both required for enhanced levels of transcription from this promoter in both N. gonorrhoeae and an Escherichia coli strain producing IHF. In addition, an UP-like element positioned between the -35 box and the IHF-binding site was required for maximal transcription. The AT-rich region upstream of the IHF-binding core consensus sequence can also act as an UP-like element when appropriately repositioned upstream of the -35 box.

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Citations

Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Patricia MartinE Richard Moxon
Feb 10, 2009·Applied and Environmental Microbiology·Michael G MorashPaul S Hoffman
Apr 28, 2009·FEMS Microbiology Reviews·Stuart A Hill, John K Davies
Feb 24, 2015·Microbiology·Jenny WachterStuart A Hill
Aug 1, 2013·Molecular Microbiology·María A RendónMagdalene So
Jan 5, 2013·Microbiology·Chantalle A J PitreAnn Karen C Brassinga

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