PMID: 9445037Jan 28, 1998Paper

An ATF/CRE element mediates both EBNA2-dependent and EBNA2-independent activation of the Epstein-Barr virus LMP1 gene promoter

Journal of Virology
A SjöblomL Rymo

Abstract

The Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) is a viral oncogene whose expression is regulated by both viral and cellular factors. EBV nuclear antigen 2 (EBNA2) is a potent transactivator of LMP1 expression in human B cells, and several EBNA2 response elements have been identified in the promoter regulatory sequence (LRS). We have previously shown that an activating transcription factor/cyclic AMP response element (ATF/CRE) site in LRS is involved in EBNA2 responsiveness. We now establish the importance of the ATF/CRE element by mutational analysis and show that both EBNA2-dependent activation and EBNA2-independent activation of the promoter occur via this site but are mediated by separate sets of factors. An electrophoretic mobility shift assay (EMSA) with specific antibodies showed that the ATF-1, CREB-1, ATF-2 and c-Jun factors bind to the site as ATF-1/CREB-1 and ATF-2/c-Jun heterodimers whereas the Sp1 and Sp3 factors bind to an adjacent Sp site. Overexpression of ATF-1 and CREB-1 in the cells by expression vectors demonstrated that homodimeric as well as heterodimeric forms of the factors transactivate the LMP1 promoter in an EBNA2-independent manner. The homodimers of ATF-2 and c-Jun did not significantl...Continue Reading

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Citations

May 16, 2008·Journal of Virology·Walter LucchesiPaul J Farrell
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bo ZhaoEllen Cahir-McFarland
Nov 16, 2004·Journal of Virology·Yao ChangChing-Hwa Tsai
Jan 15, 2005·Journal of Virology·Honglin ChenS Diane Hayward
Dec 31, 2009·Virology Journal·Jose L GarridoAdam Rosendorff
Jan 22, 2000·Journal of Virology·S ZhouS D Hayward
Dec 19, 2000·Journal of Virology·S Kusano, N Raab-Traub
Mar 13, 2009·Journal of Virology·Constantinos Demetriades, George Mosialos
Dec 8, 2017·Frontiers in Microbiology·Masahiro YoshidaHiroshi Kimura
Mar 16, 2000·Microbes and Infection·B Wensing, P J Farrell

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