PMID: 129326Jan 15, 1976

An ATPase depending on the presence of single-stranded DNA from mouse myeloma

European Journal of Biochemistry
H J Hachmann, A Lezius

Abstract

An ATPase was purified from mouse myeloma MOPC 70E the activity of which depends on the presence of single-stranded DNA and divalent cations such as Mg2+, Mn2+, Ca2+, Ni2+ or Fe2+. The enzyme splits both ribonucleoside and deoxyribonucleoside triphosphates but preferentially ATP and dATP yielding nucleoside diphosphates and inorganic phosphate. The enzyme has an absolute requirement for single-stranded DNA. Alternating double-stranded polydeoxynucleotides are only slight effective, and native double-stranded DNA, single-stranded and double-stranded RNAs as well as DNA - RNA hybrids are ineffective in stimulating the ATPase. The enzyme has further characterized by sedimentation in a sucrose density gradient (s20, w = 5.5 S) and by isoelectric focussing in an ampholine pH gradient (pI = 6.5).

References

Jan 2, 1975·European Journal of Biochemistry·H J Hachmann, A Lezius
Oct 1, 1975·Nucleic Acids Research·F EcksteinR Wetzel
Jan 1, 1973·Annual Review of Genetics·A J Clark
Oct 9, 1970·Biochemical and Biophysical Research Communications·N DebreceniK Ebisuzaki
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J R Horne, V A Erdmann
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C Lowery-Goldhammer, J P Richardson
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W Wickner, A Kornberg
Apr 2, 1973·European Journal of Biochemistry·S Dezélée, A Sentenac
Jan 1, 1974·Progress in Nucleic Acid Research and Molecular Biology·W Arber
Dec 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A Lezius, B Müller-Lornsen
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·T W CONWAY, F LIPMANN

Citations

Jul 7, 1981·Molecular and Cellular Biochemistry·R K Banerjee
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E RapaportP C Zamecnik
Aug 12, 1985·Nucleic Acids Research·U Hübscher, H P Stalder
Apr 24, 1981·Nucleic Acids Research·K Yaginuma, K Koike
May 2, 1977·European Journal of Biochemistry·R M Purkey, K Ebisuzaki
Sep 15, 1977·European Journal of Biochemistry·M Abdel-MonemH Hoffmann-Berling
Aug 15, 1979·European Journal of Biochemistry·L M Assairi, I R Johnston
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·H KatsuraK Kida
Nov 1, 1980·Journal of Cellular Physiology·E Rapaport
Jun 1, 1981·European Journal of Biochemistry·P J De JongH S Jansz

Related Concepts

DNA-dependent ATPase
Cations, Divalent
DNA, Neoplasm
DNA, Single-Stranded
Enzyme Activation
Genomic Hybridization

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