An AU-box motif upstream of the SD sequence of light-dependent psbA transcripts confers mRNA instability in darkness in cyanobacteria

Nucleic Acids Research
G K AgrawalMakoto Shirai

Abstract

The psbA2 gene of a unicellular cyanobacterium, Microcystis aeruginosa K-81, encodes a D1 protein homolog in the reaction center of photosynthetic Photosystem II. The expression of the psbA2 transcript has been shown to be light-dependent as assessed under light and dark (12/12 h) cycling conditions. We aligned the 5'-untranslated leader regions (UTRs) of psbAs from different photosynthetic organisms and identified a conserved sequence, UAAAUAAA or the 'AU-box', just upstream of the SD sequences. To clarify the role of 5'-upstream cis-elements containing the AU-box for light-dependent expression of psbA2, a series of deletion and point mutations in the region were introduced into the genome of heterologous cyanobacterium Synechococcus sp. strain PCC 7942, and psbA2 expression was examined. A clear pattern of light-dependent expression was observed in recombinant cyanobacteria carrying the K-81 psbA2 -38/+36 region (which includes the minimal promoter element and a light-dependent cis-element with the AU-box), +1 indicating the transcription start site. A constitutive pattern of expression, in which the transcripts remained almost stable under dark conditions, was obtained in cells harboring the -38/+14 region (the minimal eleme...Continue Reading

References

Jun 1, 1992·Archives of Biochemistry and Biophysics·F NilssonB Andersson
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Oct 1, 1996·Plant Molecular Biology·M Sugita, M Sugiura
Mar 20, 1999·Biochemical and Biophysical Research Communications·G K AgrawalM Shirai

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Citations

Aug 1, 2009·Cellular and Molecular Life Sciences : CMLS·Paula MuloEva-Mari Aro
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xinyao LiuRoy Curtiss
Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ryan P BezyDavid M Kehoe
Mar 27, 2012·Journal of Biotechnology·Iara M P Machado, Shota Atsumi
Dec 23, 2015·ACS Synthetic Biology·Lun YaoElton Paul Hudson
May 3, 2015·FEMS Microbiology Reviews·Matthias Kopf, Wolfgang R Hess
Mar 22, 2014·RNA Biology·Wolfgang R HessGabriele Klug
Sep 3, 2003·Bioscience, Biotechnology, and Biochemistry·Ganesh Kumar AgrawalMakoto Shirai

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