An autoantibody profile detects Brugada syndrome and identifies abnormally expressed myocardial proteins.

European Heart Journal
Diptendu ChatterjeeRobert M Hamilton

Abstract

Brugada syndrome (BrS) is characterized by a unique electrocardiogram (ECG) pattern and life-threatening arrhythmias. However, the Type 1 Brugada ECG pattern is often transient, and a genetic cause is only identified in <25% of patients. We sought to identify an additional biomarker for this rare condition. As myocardial inflammation may be present in BrS, we evaluated whether myocardial autoantibodies can be detected in these patients. For antibody (Ab) discovery, normal human ventricular myocardial proteins were solubilized and separated by isoelectric focusing (IEF) and molecular weight on two-dimensional (2D) gels and used to discover Abs by plating with sera from patients with BrS and control subjects. Target proteins were identified by mass spectrometry (MS). Brugada syndrome subjects were defined based on a consensus clinical scoring system. We assessed discovery and validation cohorts by 2D gels, western blots, and ELISA. We performed immunohistochemistry on myocardium from BrS subjects (vs. control). All (3/3) 2D gels exposed to sera from BrS patients demonstrated specific Abs to four proteins, confirmed by MS to be α-cardiac actin, α-skeletal actin, keratin, and connexin-43, vs. 0/8 control subjects. All (18/18) BrS s...Continue Reading

References

Feb 1, 1996·Journal of the American College of Cardiology·D CorradoG Thiene
Nov 27, 2002·European Heart Journal·A A M WildeUNKNOWN Study Group on the Molecular Basis of Arrhythmias of the European Society of Cardiology
Dec 14, 2002·Circulation Research·C AntzelevitchA R Perez Riera
Jan 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Steven PoelzingDavid S Rosenbaum
May 19, 2005·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Charles AntzelevitchArthur Wilde
Jul 28, 2010·Journal of Molecular and Cellular Cardiology·Arthur A M WildeCharles Antzelevitch
Sep 11, 2010·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Theano PapavassiliuMartin Borggrefe
Dec 22, 2012·Circulation Journal : Official Journal of the Japanese Circulation Society·Taisuke IshikawaAkinori Kimura
May 18, 2013·Biochimica Et Biophysica Acta·David F Steele, David Fedida
Feb 20, 2014·FEBS Letters·Esperanza Agullo-PascualMario Delmar
Aug 19, 2015·Nature Medicine·Stamatis PapathanasiouYassemi Capetanaki
Oct 31, 2015·Journal of the American College of Cardiology·Koonlawee NademaneeElijah R Behr
Jul 18, 2016·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Charles AntzelevitchArthur A M Wilde
Jan 11, 2017·Cardiovascular Research·Sarah H VermijToon A B van Veen
Jun 4, 2017·Biochimica Et Biophysica Acta. Biomembranes·Irina Epifantseva, Robin M Shaw
Sep 20, 2017·Circulation Research·Wassim A BasheerRobin M Shaw
Dec 20, 2017·Trends in Cardiovascular Medicine·Marina Cerrone
Jul 1, 2018·Circulation·S Mohsen HosseiniUNKNOWN National Institutes of Health Clinical Genome Resource Consortium
Jul 22, 2018·Biophysics Reviews·Mary TsikitisYassemi Capetanaki
Sep 22, 2018·European Heart Journal·Diptendu ChatterjeeRobert M Hamilton
Dec 1, 2018·Journal of the American College of Cardiology·Maurizio PieroniLeonardo Bolognese

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Citations

Jun 20, 2020·European Heart Journal·Arthur A M Wilde, Elisabeth M Lodder
Sep 13, 2020·European Heart Journal·Robert M HamiltonArdan M Saguner
Nov 27, 2020·Current Cardiology Reports·Jin Li
Jan 4, 2021·European Heart Journal·Harry J G M CrijnsJosep Brugada
Jan 2, 2021·International Journal of Molecular Sciences·Katarina AndelovaNarcis Tribulova
Nov 19, 2020·European Heart Journal·Arthur A M Wilde, Elisabeth M Lodder
Mar 20, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Meng-Ting LiZheng-Xin Xu
Apr 4, 2021·International Journal of Molecular Sciences·Nienke M StegeHerman H W Silljé
May 22, 2021·Journal of the American College of Cardiology·John R GiudicessiJason C Kovacic
Jul 3, 2021·Journal of Clinical Medicine·Alida L P CaforioNabeel Hamzeh

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