An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay.

Immunohematology
A D HoS R Stowell

Abstract

While a variety of therapeutic options continue to emerge for COVID-19 treatment, convalescent plasma (CP) has been used as a possible treatment option early in the pandemic. One of the most significant challenges with CP therapy, however, both when defining its efficacy and implementing its approach clinically, is accurately and efficiently characterizing an otherwise heterogenous therapeutic treatment. Given current limitations, our goal is to leverage a SARS antibody testing platform with a newly developed automated endpoint titer analysis program to rapidly define SARS-CoV-2 antibody levels in CP donors and hospitalized patients. A newly developed antibody detection platform was used to perform a serial dilution enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (Ig)G, IgM, and IgA SARS-CoV-2 antibodies. Data were then analyzed using commercially available software, GraphPad Prism, or a newly developed program developed in Python called TiterScape, to analyze endpoint titers. Endpoint titer calculations and analysis times were then compared between the two analysis approaches. Serial dilution analysis of SARS-CoV-2 antibody levels revealed a high level of heterogeneity between individuals. Commercial platform anal...Continue Reading

References

Jan 23, 1999·Journal of Immunological Methods·A FreyD Zurakowski
Nov 27, 2007·Journal of Pharmaceutical and Biomedical Analysis·J EzzelleM P D'Souza
Feb 27, 2014·Shock·Geir StrandenesEinar K Kristoffersen
Oct 2, 2014·The New England Journal of Medicine·Lars B HolstUNKNOWN Scandinavian Critical Care Trials Group
Feb 20, 2015·Hematology·Christine L Kempton, Shannon L Meeks
May 16, 2015·International Journal of Laboratory Hematology·D M Adcock, E J Favaloro
Jan 21, 2016·Blood·Connie M ArthurSean R Stowell
May 14, 2016·World Journal of Virology·Muthannan Andavar Ramakrishnan
May 24, 2016·JAMA Pediatrics·Katherine A SpargerMartha Sola-Visner
Nov 6, 2018·The New England Journal of Medicine·Anna CurleyUNKNOWN PlaNeT2 MATISSE Collaborators
May 10, 2019·Vox Sanguinis·Christopher P Stowell, Sean R Stowell
May 28, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Erica SpackmanChristopher B Stephens
Mar 3, 2020·The Lancet Infectious Diseases·Long ChenYuan Shi
Mar 5, 2020·Transfusion·Gregory A Denomme, Waseem Q Anani
Mar 29, 2020·JAMA : the Journal of the American Medical Association·Chenguang ShenLei Liu
Mar 29, 2020·JAMA : the Journal of the American Medical Association·John D Roback, Jeannette Guarner
Apr 8, 2020·The Journal of Clinical Investigation·Evan M BlochAaron Ar Tobian
May 2, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sean Stowell, Jeannette Guarner
Jun 12, 2020·The Journal of Clinical Investigation·Michael J JoynerArturo Casadevall
Jul 4, 2020·Journal of Thrombosis and Haemostasis : JTH·Katrien M J DevreeseHarlinde Peperstraete
Jul 18, 2020·The New England Journal of Medicine·UNKNOWN RECOVERY Collaborative GroupMartin J Landray
Aug 9, 2020·The Journal of Infectious Diseases·Gerco den HartogRobert S van Binnendijk
Aug 25, 2020·Cell Reports Medicine·Mehul S SutharJens Wrammert
Sep 29, 2020·EClinicalMedicine·Hans P Verkerke, Cheryl L Maier
Oct 24, 2020·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J W L AllenS R Stowell
Jan 7, 2021·The New England Journal of Medicine·Romina LibsterUNKNOWN Fundación INFANT–COVID-19 Group
Jan 8, 2021·Cell·Wilfredo F Garcia-BeltranAlejandro B Balazs
Jan 14, 2021·The New England Journal of Medicine·Louis M Katz
Jan 21, 2021·Nature·Roxanne Khamsi

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