An automated continuous flow procedure for the determination of enterokinase

Clinica Chimica Acta; International Journal of Clinical Chemistry
J P VaultierJ F Grenier

Abstract

A continuous flow method has been developed for the automatic determination of enterokinase in rat small intesstine mucosa and/or luminal content. Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation. L-benzoyl-arginine paranitroanilide was then added and split to paranitroaniline by the trypsin so formed. Liberated paranitroalinine was diazotized and converted by the Bratton-Marshall reagent (N-naphthyl ethylene diamine) to an azodye, with maximum absorption at 550 nm. This method of determination was found to be six times more sensitive than the direct p-nitroaniline determination method. 36 determinations can be made hourly.

Citations

Apr 19, 1969·Lancet·B HadornO H Wolff
Sep 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·J H MilstoneV K Milstone
Mar 20, 1939·The Journal of General Physiology·M Kunitz

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Enteropeptidase
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Intestines, Small

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