An automated device for the digitization and 3D modelling of insects, combining extended-depth-of-field and all-side multi-view imaging

ZooKeys
Bernhard StröbelMichael Heethoff

Abstract

Digitization of natural history collections is a major challenge in archiving biodiversity. In recent years, several approaches have emerged, allowing either automated digitization, extended depth of field (EDOF) or multi-view imaging of insects. Here, we present DISC3D: a new digitization device for pinned insects and other small objects that combines all these aspects. A PC and a microcontroller board control the device. It features a sample holder on a motorized two-axis gimbal, allowing the specimens to be imaged from virtually any view. Ambient, mostly reflection-free illumination is ascertained by two LED-stripes circularly installed in two hemispherical white-coated domes (front-light and back-light). The device is equipped with an industrial camera and a compact macro lens, mounted on a motorized macro rail. EDOF images are calculated from an image stack using a novel calibrated scaling algorithm that meets the requirements of the pinhole camera model (a unique central perspective). The images can be used to generate a calibrated and real color texturized 3Dmodel by 'structure from motion' with a visibility consistent mesh generation. Such models are ideal for obtaining morphometric measurement data in 1D, 2D and 3D, th...Continue Reading

References

Jan 15, 1979·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S Ullman
May 17, 2006·Trends in Ecology & Evolution·Brian J McGillMark Westoby
Sep 25, 2008·The American Naturalist·A P Møller, R Thornhill
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Oct 21, 2015·Nature Communications·Sagrario Gámez-ViruésCatrin Westphal
Nov 26, 2015·PloS One·Lawrence N HudsonVincent S Smith
May 29, 2016·Insect Science·Sara KühselNico Blüthgen

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Citations

Aug 5, 2020·American Journal of Physical Anthropology·Christopher Martin Silvester, Simon Hillson
Nov 30, 2020·Journal of Biophotonics·Meng LiMikkel Brydegaard
Jan 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Toke T HøyeJenni Raitoharju
Oct 29, 2021·PLoS Computational Biology·Eline De BorreArno Thielens

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Methods Mentioned

BETA
X-ray

Software Mentioned

PhotoScan Pro
Shotcut
Wavefront
PhotoScan
DISC3D
Agisoft PhotoScan Pro
PAST
MATLAB
Agisoft PhotoScan

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