PMID: 7541432Jun 28, 1995Paper

An automated ELISA system using a pipette tip as a solid phase and a pH-sensitive field effect transistor as a detector

Journal of Immunological Methods
H TsurutaM Nakamura

Abstract

A fully automated ELISA system was constructed using a pipette tip as a solid phase, urease as a detecting enzyme, and a pH-FET as a detector of urease activity. The inner wall of the end part of a pipette tip was used as a solid phase, and the urease activity of the conjugate, captured after a two-step immunoreaction, was measured by coupling the pipette tip with the pH-FET in a pH-measuring cell. Full automation of the ELISA system was achieved by using a disposable reagent cartridge and three pipetters for all mechanical operations, including sample dilution and B/F separation. This system can treat 60 samples per hour with an assay time of 21 min for all assay configurations. The system was applied to two-step sandwich assays for AFP, CEA, HBsAg, and HBsAb, a two-step competition assay for HBcAb, and a second antibody assay for HTLV-I Ab.

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Citations

Aug 23, 2002·Biosensors & Bioelectronics·Z Emmanuel SelvanayagamL C Ho
Jan 1, 2009·Sensors·Chang-Soo LeeMoonil Kim
Jun 22, 2005·Biosensors & Bioelectronics·Seiji TakedaKazuhiko Matsumoto
Dec 13, 2005·Biosensors & Bioelectronics·Christophe A Marquette, Loïc J Blum
Oct 12, 2002·The Analyst·Michael J Schöning, Arshak Poghossian

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