PMID: 8601321Apr 1, 1996Paper

An automated enzymatic method on the Roche COBAS MIRA S for monitoring phenylalanine in dried blood spots of patients with phenylketonuria

Clinical Biochemistry
E W Randell, D C Lehotay

Abstract

To describe and evaluate a phenylalanine dehydrogenase-coupled enzymatic assay for blood-spot phenylalanine (Phe) automated on the COBAS MIRA S analyzer for monitoring Phe in phenylketonuria (PKU) patients, as part of a home testing program. This method required a four-point calibration with each run and the useful range was 9.3 to 3500 micromol/L Phe. The within-run precision (CV%) was 8.8% at a mean of 77 micromol/L Phe and 5.3% at 787 micromol/L Phe. The between-run precision was 15% and 5.6% for 104 micromol/L and 748 micromol/L Phe, respectively. Blood-spot Phe determinations by this method were compared with plasma Phe determined by the Beckman system 7300 HPLC analyzer using 152 samples collected from PKU patients and 56 samples from patients without PKU. Linear regression analysis revealed the equation y = 0.933x + 14.9. The standard error of estimate (Sy.x) was 82.9 and the correlation coefficient (r) was 0.98. A positive bias, observed for the blood-spot Phe assay with specimens containing Phe concentrations below 200 micro mol/L, was not due to carryover or tyrosine. The results indicate that this method is acceptable for monitoring blood Phe levels in PKU patients.

References

Sep 30, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·R S CampbellC P Price
Dec 16, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y QuE Shapira
Nov 1, 1974·Annals of Clinical Biochemistry·P M BroughtonD W Neill
Mar 1, 1994·Annals of Clinical Biochemistry·R S CampbellC P Price
Sep 30, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·R P TaylorS J Standing
Jan 1, 1983·Journal of Pharmaceutical and Biomedical Analysis·W T KokR W Frei

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Citations

Dec 27, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Sadeq Vallian, Hassan Moeini
Jun 12, 2021·Diagnostic Microbiology and Infectious Disease·Coleman T TurgeonElitza S Theel

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