An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli

Uwe RiekDietbert Neumann


We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.


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Dec 14, 1999·Protein Science : a Publication of the Protein Society·M EderK Fritz-Wolf
Oct 3, 2002·Protein Expression and Purification·Michael A ThielKeryn A Williams
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Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Darren J Hart, Franck Tarendeau
Jun 20, 2006·Current Opinion in Biotechnology·Dominic Esposito, Deb K Chatterjee

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Feb 19, 2010·The Journal of Biological Chemistry·Enea SalsiAndrea Mozzarelli
May 21, 2014·ELife·David DegenRichard H Ebright

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