An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli

BioTechniques
Uwe RiekDietbert Neumann

Abstract

We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.

References

Apr 1, 1996·Current Opinion in Biotechnology·G Georgiou, P Valax
Oct 1, 1996·Current Opinion in Biotechnology·M J WeickertP O Olins
Dec 14, 1999·Protein Science : a Publication of the Protein Society·M EderK Fritz-Wolf
Oct 3, 2002·Protein Expression and Purification·Michael A ThielKeryn A Williams
Aug 25, 2004·Methods : a Companion to Methods in Enzymology·Zygmunt S Derewenda
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Darren J Hart, Franck Tarendeau
Jun 20, 2006·Current Opinion in Biotechnology·Dominic Esposito, Deb K Chatterjee

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Citations

Feb 19, 2010·The Journal of Biological Chemistry·Enea SalsiAndrea Mozzarelli
May 21, 2014·ELife·David DegenRichard H Ebright

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