PMID: 9654243Jul 8, 1998Paper

An automated method for discriminating aneugen- vs. clastogen-induced micronuclei

Environmental and Molecular Mutagenesis
Dorothea K TorousC R Tometsko

Abstract

A flow cytometric (FCM) procedure for quantitating micronucleated reticulocytes in mouse peripheral blood samples was evaluated for its ability to discriminate between aneugen- and clastogen-induced micronuclei (MN). In this experiment, BALB/c mice were injected with 0.9% saline, the model clastogen methyl methanesulfonate (100 mg/kg bw) or the aneugen vincristine (0.2 mg/kg bw). Peripheral blood samples were collected 48 hr after injection and were subsequently fixed and stained for flow cytometric analysis. The staining method utilized FITC-conjugated anti-CD71 to differentially label reticulocytes, and the nucleic acid dye propidium iodide to resolve erythrocyte populations with and without micronuclei. The frequency of micronucleated reticulocytes was determined by analyzing 10,000 total reticulocytes per blood sample. A second analysis was performed on each sample whereby the propidium iodide associated fluorescent signals of 250 MN were collected and graphed as a single-parameter histogram. The histogram statistic "median channel" was recorded for each sample and provided a quantitative description of MN distribution according to DNA content. Cumulatively, the results of this study suggest that 1) flow cytometry can be em...Continue Reading

Citations

Apr 6, 2004·Environmental and Molecular Mutagenesis·Rocío OrtizEdith Cortés
Nov 29, 2011·Mutation Research·Elsa Cervantes-RíosLeonor Rodríguez-Cruz
Feb 22, 2012·Environmental and Molecular Mutagenesis·Cheryl A HobbsKristine L Witt
Oct 1, 2009·Environmental and Molecular Mutagenesis·Libo LiuShuzheng Liu
Jul 25, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N KotovaJ Grawé
Jun 14, 2003·Environmental Research·S R C SoaresP H N Saldiva
Sep 29, 2005·Radiation Research·Polly Y ChangRupa Doppalaudi

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