An automated method for measuring lipoprotein lipase and hepatic triglyceride lipase activities in post-heparin plasma

Clinica Chimica Acta; International Journal of Clinical Chemistry
Kiyomi NakajimaMasami Murakami

Abstract

Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) play a central role in triglyceride-rich lipoprotein metabolism by catalyzing the hydrolysis of triglycerides. Quantification of LPL and HTGL activity is useful for diagnosing lipid disorders, but there has been no automated method for measuring these lipase activities. The automated kinetic colorimetric method was used for assaying LPL and HTGL activity in the post-heparin plasma using the natural long-chain fatty acid 2-diglyceride as a substrate. LPL activity was determined with apoCII and HTGL activity was determined without apoCII with 2 channel of auto-analyzer. The calibration curve for dilution tests of the LPL and HTGL activity assay ranged from 0.0 to 500 U/L. Within-run CV was obtained within a range of 5%. No interference was observed in the testing of specimens containing potentially interfering substances. The measurement range of LPL activity in the post-heparin plasma was 30-153 U/L, while HTGL activity was 135-431 U/L in normal controls. The L PL and HTGL activity assays are applicable to quantitating the LPL and HTGL activity in the post-heparin plasma. This assay is more convenient and faster than radiochemical assay and highly suitable for the d...Continue Reading

Citations

May 26, 2020·Journal of Atherosclerosis and Thrombosis·Haizhao YanJianglin Fan
Aug 8, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Haizhao YanJianglin Fan

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