An automated method for the analysis of food intake behaviour in Caenorhabditis elegans

Scientific Reports
Mª Jesús Rodríguez-PaleroMarta Artal-Sanz

Abstract

The study of mechanisms that govern feeding behaviour and its related disorders is a matter of global health interest. The roundworm Caenorhabditis elegans is becoming a model organism of choice to study these conserved pathways. C. elegans feeding depends on the contraction of the pharynx (pumping). Thanks to the worm transparency, pumping can be directly observed under a stereoscope. Therefore, C. elegans feeding has been historically investigated by counting pharyngeal pumping or by other indirect approaches. However, those methods are short-term, time-consuming and unsuitable for independent measurements of sizable numbers of individuals. Although some particular devices and long-term methods have been lately reported, they fail in the automated, scalable and/or continuous aspects. Here we present an automated bioluminescence-based method for the analysis and continuous monitoring of worm feeding in a multi-well format. We validate the method using genetic, environmental and pharmacological modulators of pharyngeal pumping. This flexible methodology allows studying food intake at specific time-points or during longer periods of time, in single worms or in populations at any developmental stage. Additionally, changes in feed...Continue Reading

References

Mar 1, 1990·The Journal of Experimental Zoology·L Avery, H R Horvitz
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
May 28, 1982·Science·H R HorvitzP D Evans
Jun 19, 2001·Journal of Inherited Metabolic Disease·P E Kuwabara, N O'Neil
Dec 13, 2002·The Journal of Experimental Biology·Timothy Niacaris, Leon Avery
Feb 1, 2003·Experimental Gerontology·Koen HouthoofdJacques R Vanfleteren
Jun 11, 2003·The Journal of Experimental Biology·Leon Avery, Boris B Shtonda
Sep 27, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Koen HouthoofdJacques R Vanfleteren
Dec 16, 2005·The Journal of Experimental Biology·Boris Borisovich Shtonda, Leon Avery
Nov 14, 2006·Aging Cell·Garrick D LeeSige Zou
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Daniel L Chase, Michael R Koelle
Aug 6, 2008·Cell Metabolism·Matthew D W Piper, Andrzej Bartke
Feb 26, 2009·Genes & Development·Alexander A SoukasGary Ruvkun
Mar 21, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cristina LagidoL Anne Glover
May 2, 2009·Disease Models & Mechanisms·Kevin T Jones, Kaveh Ashrafi
Jun 4, 2010·Antioxidants & Redox Signaling·Leanne M Redman, Eric Ravussin
Apr 20, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Shuhei SoYasumi Ohshima
Jun 8, 2011·PloS One·Daniel D Shaye, Iva Greenwald
Feb 11, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bo-mi Song, Leon Avery
Mar 10, 2012·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Paige M DexterGuy A Caldwell
Jan 19, 2013·Animal Cells and Systems·Young-Jai You, Leon Avery
Sep 23, 2014·EMBO Reports·Nilay YapiciAna I Domingos
Jan 17, 2015·Cell·George A LemieuxKaveh Ashrafi
Mar 31, 2015·Cell·Luigi Fontana, Linda Partridge
Apr 22, 2015·ELife·Mi Cheong CheongLeon Avery
Apr 24, 2015·Genetics·Rafael L Gomez-AmaroMichael Petrascheck
Oct 17, 2015·Annual Review of Genetics·George A Lemieux, Kaveh Ashrafi
Oct 31, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicolas DallièreVincent O'Connor
Mar 5, 2016·WormBook : the Online Review of C. Elegans Biology·Michael R Koelle
Apr 22, 2016·Scientific Reports·Moonjung HyunYoung-Jai You
May 20, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Holly MillsRichard Komuniecki
Jun 13, 2016·Trends in Endocrinology and Metabolism : TEM·George A Lemieux, Kaveh Ashrafi
Jul 31, 2016·Journal of Neuroscience Methods·Monika ScholzDavid Biron
Feb 23, 2017·Frontiers in Neuroscience·Aurea Blancas-VelazquezSusanne E la Fleur
Mar 16, 2017·Neuroscience and Biobehavioral Reviews·Maria RazzoliAlessandro Bartolomucci
Jun 16, 2017·Nature Reviews. Disease Primers·Pedro González-MuniesaJ Alfredo Martinez
Jul 13, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthew A ChurginChristopher Fang-Yen

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Citations

Dec 12, 2019·Environmental Science and Pollution Research International·Ying-Fei YangChung-Min Liao
Jul 8, 2020·Lab on a Chip·Vittorio ViriMartin A M Gijs
Jul 2, 2021·Toxicology Reports·Fernando CalahorroVincent O'Connor
Aug 20, 2021·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Abdullah AlmotayriMarkandeya Jois

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Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.