An automated microassay for blood ammonia

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
M ImlerA Stahl


A microassay for blood ammonia has been developed. After dilution, blood is dialyzed by a continuous flow procedure, and the ammonium ions in the dialysate are assayed colorimetrically by the hypochlorite-phenol Berthelot reaction. Concentrations from 1 to 400 mumol/l NH+4 can be measured in a 50 mul whole blood sample. Using slight modifications, concentrations up to 800 mumol/l NH+4 can be determined. The precision, accuracy, sensitivity, and specificity of the method have been studied. In man, the usual values (mean +/- 2 SD) found are 27.4 +/- 10.2 mumol/l NH+4 in arterial blood, 20.9 +/- 9.8 mumol/l in venous blood. The usual values in rats and mice are very close to those given for man.


Mar 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·M ImlerJ Stahl


Jan 1, 1988·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M ImlerJ L Schlienger
Jan 1, 1986·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M ImlerC Simon
Feb 18, 1983·European Journal of Pharmacology·J M WarterJ Krieger
Apr 16, 1986·European Journal of Pharmacology·M ImlerJ M Warter
Jul 1, 1985·Journal of the Neurological Sciences·J M WarterM Imler
Feb 13, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M JózwikM Szamatowicz
Dec 4, 2008·The FEBS Journal·Daniela VannoniEnrico Marinello
Feb 8, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Geert A A NibourgRobert A F M Chamuleau
Sep 20, 2017·Organic & Biomolecular Chemistry·Yoshinori YamamotoKeiko Yamamoto

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