Abstract
Caenorhabditis elegans (C. elegans) is a widely used animal model to study mechanisms of biological processes and human diseases. To facilitate manipulations of C. elegans in the laboratory, researchers have developed various tools that permit careful monitoring of behavior and changes in cellular processes. Earlier, we had reported a novel microfluidic assay device to study the neuronal basis of movement and to investigate the effects of cellular and environmental factors that can induce degeneration in certain neurons leading to movement disorder. The system involved the use of an electric field to perform electrotaxis assays, which allows detailed examination of movement responses of animals. One of the potential uses of this system is to perform genetic and chemical screenings for neuroprotective factors; however, it could not be done due to manual operations and low throughput. In this paper, we present an integrated microfluidic system that automates screening of C. elegans behavioral response using electrotaxis. The core component of system is a multilayer poly dimethyl siloxane (PDMS) device, which enables C. elegans loading, capture, flush, release, electrotaxis, and clean sequentially with the help of other components...Continue Reading
References
Dec 16, 1998·Science·C I Bargmann
Feb 3, 1999·Annual Review of Genetics·D L PriceS S Sisodia
Jan 14, 2000·Electrophoresis·J C McDonaldG M Whitesides
Sep 28, 2002·Science·Todd ThorsenStephen R Quake
May 5, 2006·Nature Reviews. Drug Discovery·Titus Kaletta, Michael O Hengartner
Sep 1, 2006·Analytical Biochemistry·Ann K Corsi
Feb 3, 2007·Lab on a Chip·Jianhua Qin, Aaron R Wheeler
Jul 13, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher V GabelAravinthan D T Samuel
Aug 21, 2007·Nature Methods·Nikos ChronisCornelia I Bargmann
Aug 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christopher B RohdeMehmet Fatih Yanik
Jun 24, 2008·Nature Methods·Kwanghun ChungHang Lu
Sep 27, 2008·Lab on a Chip·Weiwei ShiBingcheng Lin
Jan 13, 2010·Lab on a Chip·Pouya RezaiBhagwati P Gupta
Jun 16, 2010·Neuron·Ted M DawsonValina L Dawson
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chrysanthi SamaraMehmet Fatih Yanik
Jan 18, 2011·Nature Methods·Jeffrey N StirmanHang Lu
Jun 15, 2011·Nature Methods·Dirk R Albrecht, Cornelia I Bargmann
Jul 21, 2011·Biomicrofluidics·Archana ParasharSantosh Pandey
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andrés Vidal-GadeaJonathan T Pierce-Shimomura
Mar 31, 2012·Lab on a Chip·Pouya RezaiBhagwati P Gupta
Aug 21, 2012·Nature Methods·Matthew M CraneHang Lu
Jul 5, 2013·BMC Neuroscience·Aylia MohammadiWilliam S Ryu
Sep 24, 2013·Worm·Sangeena SalamBhagwati P Gupta
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Johannes LarschDirk R Albrecht
Apr 23, 2014·Biomicrofluidics·Guillaume Aubry, Hang Lu
Dec 4, 2014·PloS One·Sertan Kutal GokceAdela Ben-Yakar
Citations
Sep 8, 2017·Sensors·Yung-Shin Sun
Aug 26, 2017·Lab on a Chip·Matteo CornagliaMartin A M Gijs
Aug 8, 2020·Micromachines·Khaled YoussefPouya Rezai
Nov 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniel Midkiff, Adriana San-Miguel
Mar 15, 2020·Micromachines·Reza GhaemiP Ravi Selvaganapathy
Jul 19, 2016·Micromachines·Bhagwati P Gupta, Pouya Rezai
Dec 11, 2020·The Science of the Total Environment·Yutao BaiDonald Wlodkowic