An automated platform for the enzyme-mediated assembly of complex oligosaccharides

Nature Chemistry
Tiehai LiGeert-Jan Boons

Abstract

An automated platform that can synthesize a wide range of complex carbohydrates will greatly increase their accessibility and should facilitate progress in glycoscience. Here we report a fully automated process for enzyme-mediated oligosaccharide synthesis that can give easy access to different classes of complex glycans including poly-N-acetyllactosamine derivatives, human milk oligosaccharides, gangliosides and N-glycans. Our automated platform uses a catch and release approach in which glycosyltransferase-catalysed reactions are performed in solution and product purification is accomplished by solid phase extraction. We developed a sulfonate tag that can easily be installed and enables highly efficient solid phase extraction and product release using a single set of washing conditions, regardless of the complexity of the glycan. Using this custom-built synthesizer, as many as 15 reaction cycles can be performed in an automated fashion without a need for lyophilization or buffer exchange steps.

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Citations

Jul 13, 2019·Faraday Discussions·Marco MendePeter H Seeberger
Jul 25, 2019·Organic & Biomolecular Chemistry·Ingrid M E 't HartGeert-Jan Boons
Dec 19, 2019·Molecular & Cellular Proteomics : MCP·Brian B Haab, Zachary Klamer
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Oct 14, 2020·Chemistry : a European Journal·Javier Sastre TorañoGeert-Jan Boons
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Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Brian B Haab, Zachary Klamer
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Sep 15, 2021·Angewandte Chemie·Asuka ShirakawaKoichi Fukase
Dec 24, 2021·Glycobiology·Hanna JongTom Wennekes

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Methods Mentioned

BETA
glycosylations
size exclusion column chromatography
glycosylation
column chromatography

Software Mentioned

AutoSuite

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