An automated platform to monitor long-term behavior and healthspan in Caenorhabditis elegans under precise environmental control.

Communications Biology
Kim N LeHang Lu

Abstract

Health and longevity in all organisms are strongly influenced by the environment. To fully understand how environmental factors interact with genetic and stochastic factors to modulate the aging process, it is crucial to precisely control environmental conditions for long-term studies. In the commonly used model organism Caenorhabditis elegans, existing assays for healthspan and lifespan have inherent limitations, making it difficult to perform large-scale longitudinal aging studies under precise environmental control. To address these constraints, we developed the Health and Lifespan Testing Hub (HeALTH), an automated, microfluidic-based system for robust longitudinal behavioral monitoring. Our system provides long-term (i.e. entire lifespan) spatiotemporal environmental control. We demonstrate healthspan and lifespan studies under a variety of genetic and environmental perturbations while observing how individuality plays a role in the aging process. This system is generalizable beyond aging research, particularly for short- or long-term behavioral assays, and could be adapted for other model systems.

References

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Citations

Jul 30, 2020·Pharmaceuticals·Sven Bulterijs, Bart P Braeckman
Mar 3, 2021·Lab Animal·Cassandra BackesFilipe Cabreiro
Nov 2, 2021·Disease Models & Mechanisms·Ineke DhondtWinnok H De Vos

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Software Mentioned

MATLAB script
LASSO
MATLAB
JMP Pro14
GraphPad Prism
lme4
HeALTH
R
FuDR

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