PMID: 8601318Apr 1, 1996Paper

An automated, specific, spectrophotometric method for measuring ascorbic acid in plasma (EFTSA)

Clinical Biochemistry
I F Benzie

Abstract

To evaluate an automated enzyme linked, ferric-tripyridyltriazine spectrophotometric assay (EFTSA) for plasma ascorbic acid. Using aqueous ascorbic acid solutions and plasma containing native and/or added ascorbic acid, the following were assessed: reaction kinetics, dose response relationships, recovery of added ascorbic acid, specificity, precision. Performing the test on a Cobas Fara centrifugal analyser, the test is linear at least to 400 micromol/L; within-run CVs at 20, 50, 140, and 300 micromol/L ascorbic acid, in both pure aqueous solutions and in plasma, were <5.5%; 99-105% of added ascorbic acid (from 30-130 micromol/L) was recovered. The reaction of ascorbic acid is virtually instantaneous; other native antioxidants do not appear to interfere, and there is no interference by dehydroascorbic acid when readings are taken within a 15-60-s reaction time window. EFTSA appears suitable for the routine measurement of ascorbic acid in plasma.

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Citations

Nov 22, 2011·Archives of Toxicology·İlhami Gülçin
Feb 19, 2002·Clinical Biochemistry·W Y ChungI F Benzie
Nov 13, 2004·The Journal of Nutritional Biochemistry·I F BenzieJ J Strain
Oct 22, 2002·Journal of Pharmacological and Toxicological Methods·François GaboriauJacques Philippe Moulinoux
Aug 31, 1999·The Proceedings of the Nutrition Society·I F Benzie
Sep 1, 1999·Asia Pacific Journal of Clinical Nutrition·I Benzie, J J Strain
Aug 5, 1999·Kidney International·T Nguyen-KhoaB Descamps-Latscha
Jun 1, 1997·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·H ShichiD H Shin
Apr 29, 2009·Nutrition·Kyung-Jin YeumGiancarlo Aldini
Aug 1, 1997·Redox Report : Communications in Free Radical Research·I F Benzie, J J Strain
Apr 13, 2012·Oxidative Medicine and Cellular Longevity·Deqing WangBrian Tomlinson
Jun 14, 2008·Nutrition·Graciela E Hurtado de CatalfoCarlos A Marra
Oct 19, 2016·Preventive Nutrition and Food Science·Su Jin KangJong-Sang Kim
Jul 4, 2003·Medicine and Science in Sports and Exercise·John C QuindryCraig E Broeder
Feb 3, 2009·Journal of Agricultural and Food Chemistry·Joon-Kwan Moon, Takayuki Shibamoto
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ifeanyi D NwachukwuRotimi E Aluko
Sep 1, 2021·Critical Care : the Official Journal of the Critical Care Forum·Sander RozemeijerAngélique M E de Man

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