An automated superfusion technique for measuring circular muscle contraction. Effects of serotonin on intra- and extracranial arteries

European Journal of Pharmacology
J C LamarA M Van Delft


A new in vitro technique is described and its advantages are demonstrated: "true" circular contraction is measured; arteries are in cascade, permitting comparison of intra- and extracranial arteries from the same animal; the mechanical influences on contractility are reduced; long-lasting experiments of up to 50 h can be performed; the procedure is fully automated. The maximal effect of an agonist was not only markedly modified during the first 2--4 h after fixing the arteries in the apparatus but also slightly during the next 3--4 h. After the initial stabilization period, the arterial response to an agonist remained highly reproducible for 24--48 h (standard deviation not exceeding 7%). This was demonstrated in all the types of arteries tested. With serotonin as agonist, there was a significant difference in --log ED50 values for intracranial arteries (basilar 8.70, middle cerebral 8.72) vs. extracranial (lateral nasal 8.15; facial 8.14) and peripheral (branch of saphenous 8.14) arteries.


Jun 1, 1977·The Journal of Pharmacy and Pharmacology·H BultI L Bonta
Sep 1, 1977·Agents and Actions·E Müller-Schweinitzer, H Weidmann
Sep 1, 1977·Headache·J Edmeads
Feb 25, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·E Müller-Schweinitzer
Sep 1, 1953·British Journal of Pharmacology and Chemotherapy·J H GADDUM
Jul 1, 1964·Neurology·H T DUKES, R G VIETH
Oct 1, 1964·British Journal of Pharmacology and Chemotherapy·J R VANE


Dec 20, 1979·European Journal of Pharmacology·J C Lamar, J E Hardebo

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