PMID: 11607251Jan 15, 1992Paper

An auxin-binding protein is localized to the plasma membrane of maize coleoptile cells: identification by photoaffinity labeling and purification of a 23-kda polypeptide

Proceedings of the National Academy of Sciences of the United States of America
J FeldwischK Palme

Abstract

Plasma membrane vesicles were isolated from maize (Zea mays L.) coleoptile tissue by aqueous two-phase partitioning and assayed for homogeneity by the use of membrane-specific enzymatic assays. Using 5-azido-[7-3H]indole-3-acetic acid ([3H]N3IAA), we identified several IAA-binding proteins with molecular masses of 60 kDa (pm60), 58 kDa (pm58), and 23 kDa (pm23). Using Triton X-114, we were able to selectively extract pm23 from the plasma membrane. We show that auxins and functional analogues compete with [3H]N3IAA for binding to pm23. We found that PAB130, a polyclonal antibody raised against auxin-binding protein 1 (ABP-1), recognized ABP-1 as well as pm23. This suggests that pm23 shares common epitopes with ABP-1. In addition, we identified an auxin-binding protein with a molecular mass of 24 kDa (pm24), which was detected in microsomal but not in plasma membrane vesicle preparations. Like pm23 this protein was extracted from membrane vesicles with Triton X-114. We designed a purification scheme allowing simultaneous purification of pm23 and pm24. Homogeneous pm23 and pm24 were obtained from coleoptile extracts after 7000-fold purification.

References

Feb 1, 1991·Mechanisms of Development·K PalmeJ Schell
Dec 1, 1990·Trends in Biochemical Sciences·H R Pelham
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N InoharaM Futai
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G R HicksT L Lomax
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M Jones, M A Venis
Jun 1, 1975·Plant Physiology·N J Leonard, J C Greenfield
Jun 1, 1985·Plant Physiology·S Mandala, L Taiz

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Citations

Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Estelle
Dec 1, 1994·Plant Molecular Biology·L HobbieM Estelle
Dec 1, 1994·Plant Molecular Biology·R Hooley
Aug 31, 2001·Bioorganic & Medicinal Chemistry Letters·Y TodorokiN Hirai
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·A LovegroveR Hooley
Feb 1, 1995·The New Phytologist·Richard M Napier, Michael A Venis

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