PMID: 9420224Jan 7, 1998Paper

An avirulent mutant of rabies virus is unable to infect motoneurons in vivo and in vitro

Journal of Virology
P CoulonC Tuffereau

Abstract

An antigenic double mutant of rabies virus (challenge virus standard [CVS] strain) was selected by successive use of two neutralizing antiglycoprotein monoclonal antibodies, both specific for antigenic site III. This mutant differed from the original virus strain by two amino acid substitutions in the ectodomain of the glycoprotein. The lysine in position 330 and the arginine in position 333 were replaced by asparagine and methionine, respectively. This double mutant was not pathogenic for adult mice. When injected intramuscularly into the forelimbs of adult mice, this virus could not penetrate the nervous system, either by the motor or by the sensory route, while respective single mutants infected motoneurons in the spinal cord and sensory neurons in the dorsal root ganglia. In vitro experiments showed that the double mutant was able to infect BHK cells, neuroblastoma cells, and freshly prepared embryonic motoneurons, albeit with a lower efficiency than the CVS strain. Upon further incubation at 37 degrees C, the motoneurons became resistant to infection by the mutant while remaining permissive to CVS infection. These results suggest that rabies virus uses different types of receptors: a molecule which is ubiquitously expresse...Continue Reading

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Citations

Nov 5, 2004·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·A VosA C Jackson
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Feb 8, 2011·Archives of Virology·Takashi MatsumotoAkira Nishizono
Apr 25, 2019·NPJ Vaccines·Rohan KeshwaraMatthias J Schnell
Oct 12, 2007·Journal of Virology·Yvonne KlingenStefan Finke
Jan 16, 2009·Epidemiology and Infection·W MarkotterL H Nel
Oct 19, 2011·Biotechnology Research International·Shukra M AavulaSrinivasan Alwar Villuppanoor
Feb 23, 2012·Viruses·Aurélie A V AlbertiniYves Gaudin
Mar 10, 2020·PLoS Pathogens·Laura BelotAurélie A Albertini
Aug 7, 2004·The Journal of Veterinary Medical Science·Go SatoTakeo Sakai
Nov 10, 2013·PLoS Neglected Tropical Diseases·Joe KgaladiLouis H Nel
Aug 25, 2009·Archives of Virology·Nobuyuki MochizukiTakeo Sakai
May 30, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun LuoXiaofeng Guo
Mar 4, 2011·Journal of Veterinary Science·Dong-Kun YangJae-Young Song
Dec 16, 1998·Journal of Virology·C JalletP Perrin
Jun 23, 2018·Zoonoses and Public Health·Emelissa J MendozaDavid Safronetz
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Apr 4, 2021·Viruses·Jessica CoertseWanda Markotter
Jan 10, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Angélica Cristine de Almeida CamposSilvana Regina Favoretto Lazarini

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