An axial periodic fibrillar arrangement of antigenic determinants for fibronectin and procollagen on ascorbate treated human fibroblasts

Journal of Supramolecular Structure
L T FurchtJ M Foidart

Abstract

Fibronectin and collagens are major constituents of the cell matrix of fibroblasts. Fibronectin is a 220,000 dalton glycoprotein that mediates a variety of adhesive functions of cells examined in vitro. Fibronectin is secreted in a soluble form and interacts with collagen to form extracellular filaments. Fibronectin and procollagen type I were localized using the peroxidase anti-peroxidase method. Under standard culture conditions, fibronectin and procollagen were localized to non-periodic 10 nm extracellular fibrils, the cell membrane and plasma membrane vesicles. Ascorbate treatment of cells leads to a new larger fibril with a diameter of approximately 40 nm. Antibodies to fibronectin and procollagen I react to these native collagen fibrils with an axial periodicity of approximately 70 nm. Fibronectin is clearly associated with native collagen fibrils produced by ascorbate treated cells and there is an asymetric distribution or segregation of fibronectin on these collagen fibrils with a 70 nm axial repeat.

References

Sep 1, 1979·The Journal of Clinical Investigation·D F Mosher, P E Schad
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y S Kanwar, M G Farquhar
Jan 1, 1978·International Review of Cytology·F Grinnell
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A VaheriR Timpl
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R O Hynes, A Destree
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R R BrunsJ Gross
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L H Hahn, K M Yamada
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P Bornstein, J F Ash
Mar 1, 1978·The Journal of Cell Biology·K HedmanJ Wartiovaara
Nov 1, 1978·Cell·R O Hynes, A T Destree
Jun 1, 1978·The Journal of Experimental Medicine·E EngvallE J Miller
Mar 28, 1978·Biochimica Et Biophysica Acta·W DessauH Hörmann
Nov 30, 1978·The New England Journal of Medicine·J M FoidartS I Katz
Oct 1, 1978·Journal of Ultrastructure Research·C CintronD J Cannon
Apr 30, 1976·Biochimica Et Biophysica Acta·R O Hynes
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R L TrelstadJ Gross
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S GayK Kuhn
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B D SmithG R Martin
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B R Olsen, D J Prockop
Jan 1, 1974·Annual Review of Biochemistry·P Bornstein
Dec 4, 1972·Biochemical and Biophysical Research Communications·B Peterkofsky
Dec 10, 1971·European Journal of Biochemistry·A LenaersC M Lapière
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C M LapièreL D Kohn
Jan 1, 1968·The Anatomical Record·F N Low
May 1, 1980·Analytical Biochemistry·S I RennardP G Robey
Jul 1, 1964·The Journal of Cell Biology·B GOLDBERG, H GREEN

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Citations

Jan 1, 1984·Molecular and Cellular Biochemistry·D F Mosher
Oct 15, 1984·Mechanisms of Ageing and Development·G F Edick, A J Millis
Nov 1, 1985·Collagen and Related Research·R G PhelpsB D Goldberg
Jun 1, 1993·The Journal of Cell Biology·B J DzambaD M Peters
Jan 1, 1982·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M A GibertM Pavans de Ceccatty
Nov 1, 1985·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·E RuoslahtiM D Pierschbacher
Aug 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A GeertsE Wisse
Apr 1, 1984·The Journal of Pathology·A J D'Ardenne, J O McGee
Jun 1, 1983·Journal of Ultrastructure Research·P J Courtoy, J Boyles
Nov 1, 1985·Journal of the American Academy of Dermatology·R A Clark

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