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An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells

ACS Synthetic Biology

Jan 20, 2012

Jonghyeon Shin, Vincent Noireaux

Abstract

Cell-free protein synthesis is becoming a powerful technique to construct and to study complex informational processes in vitro. Engineering synthetic gene circuits in a test tube, however, is seriously limited by the transcription repertoire of modern cell-free systems, composed of onl...read more

Mentioned in this Paper

Transcriptional Regulation
Alkalescens-Dispar Group
Bacteriophages
Protein Biosynthesis
Synthetic Genes
Vesicle
RNA Polymerase Sigma Factor H
Phospholipids
Biochemical Turnover
Phospholipid Measurement
9
382
Paper Details
References
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  • Citations84
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  • References21
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An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells

ACS Synthetic Biology

Jan 20, 2012

Jonghyeon Shin, Vincent Noireaux

PMID: 23651008

DOI: 10.1021/sb200016s

Abstract

Cell-free protein synthesis is becoming a powerful technique to construct and to study complex informational processes in vitro. Engineering synthetic gene circuits in a test tube, however, is seriously limited by the transcription repertoire of modern cell-free systems, composed of onl...read more

Mentioned in this Paper

Transcriptional Regulation
Alkalescens-Dispar Group
Bacteriophages
Protein Biosynthesis
Synthetic Genes
Vesicle
RNA Polymerase Sigma Factor H
Phospholipids
Biochemical Turnover
Phospholipid Measurement
9
382

Similar Papers Found In These Feeds

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

Artificial Cell Models

This feed focuses on biomimetrics, synthetic biology and bio- and tissue-engineering approaches used for modeling human diseases.

Related Papers

Nucleic Acids Research

Expression optimization and synthetic gene networks in cell-free systems

Nucleic Acids ResearchDecember 20, 2011
David K KarigMitchel J Doktycz
Journal of Biological Engineering

Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70

Journal of Biological EngineeringJune 26, 2010
Jonghyeon Shin, Vincent Noireaux
Paper Details
References
  • References21
  • Citations84
123
  • References21
  • Citations84
12345...
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